PIP4K2B Protein, Human (His)
PIP4K2B protein actively synthesizes phosphatidylinositol 4,5-bisphosphate and preferentially selects GTP over ATP in PI(5)P phosphorylation. The enzyme activity is related to physiological GTP levels. Its unique GTP-sensing ability is critical for metabolic adaptation. PIP4K2B Protein, Human (His) is the recombinant human-derived PIP4K2B protein, expressed by E. coli , with N-10*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
PIP4K2B protein actively synthesizes phosphatidylinositol 4,5-bisphosphate and preferentially selects GTP over ATP in PI(5)P phosphorylation. The enzyme activity is related to physiological GTP levels. Its unique GTP-sensing ability is critical for metabolic adaptation. PIP4K2B Protein, Human (His) is the recombinant human-derived PIP4K2B protein, expressed by E. coli , with N-10*His labeled tag.
Background
PIP4K2B Protein actively participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate, demonstrating a preference for GTP over ATP in the phosphorylation of PI(5)P, and its enzymatic activity is intricately linked to physiological GTP concentrations. The protein's unique GTP-sensing ability plays a critical role in metabolic adaptation. Notably, PIP4Ks, including PIP4K2B, exert a negative regulatory influence on insulin signaling through a catalytic-independent mechanism. They form interactions with PIP5Ks, effectively suppressing PIP5K-mediated synthesis of PtdIns(4,5)P2 and impeding insulin-dependent conversion to PtdIns(3,4,5)P3. This multifaceted role underscores the importance of PIP4K2B in modulating key signaling pathways that contribute to cellular homeostasis and metabolic responsiveness.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His
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Accession
P78356 (S2-T416)
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Molecular Construction
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N-term
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10*His
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PIP4K2B (S2-T416)
Accession # P78356 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
PIP4K2B; Diphosphoinositide Kinase 2-Beta; Prev. PIP5K2B; PIP4KII-Beta; PIP5KIIbeta; Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Beta; PIP5KIIB; 1-Phosphatidylinositol-4-Phosphate Kinase; Phosphatidylinositol-4-Phosphate 5-Kinase, Type II, Beta; P
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AA Sequence
SSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMDYSLLVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGTPPDSPGNLLSFPRFFGPGEFDPSVDVYAMKSHESSPKKEVYFMAIIDILTPYDTKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFNEFMSNILT
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Predicted Molecular Mass
53.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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