PKCi Protein, Human (H114A, His)

PKCi protein displays versatile enzymatic activities, functioning as an adenosine 5'-monophosphoramidase capable of hydrolyzing purine nucleotide phosphoramidates, such as adenosine 5'-monophosphoramidate (AMP-NH2), to generate AMP and NH2. It also exhibits hydrolytic activity towards adenosine 5'-monophosphomorpholidate (AMP-morpholidate), guanosine 5'-monophosphomorpholidate (GMP-morpholidate), lysyl-AMP, Met-AMP, His-AMP, Asp-AMP, lysyl-GMP, and AMP-N-alanine methyl ester. Moreover, PKCi participates in the conversion of adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates, releasing hydrogen sulfide in the process. Beyond its enzymatic functions, PKCi serves as a scaffolding protein, modulating transcriptional activation by the LEF1/TCF1-CTNNB1 complex and the MITF-CTNNB1 complex. Additionally, it influences p53/TP53 levels, p53/TP53-mediated apoptosis, and regulates the proteasomal degradation of target proteins through the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Furthermore, PKCi exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17 and RANGAP1. This multifaceted functionality underscores the diverse roles of PKCi in various cellular processes.