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PKM2 Protein, Human (420a.a, His)

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Based on 1 publication(s) in Google Scholar

PKM2 Protein, Human (420a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways.

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  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for PKM2 Protein, Human (420a.a, His)
  • PKM2 Protein, Human (420a.a, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

PKM2 Protein, Human (420a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways[1].

Background

Pyruvate Kinase M2 (PKM2), a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 is a ubiquitous prototype enzyme present in all tissues during the embryonic stage and is gradually replaced by other isozymic forms in specific tissues during development. Although the primary function of PKM2 is to catabolize glucose, it is involved in many nonglycolytic pathways, influencing the cellular physiology[1].

Verified Bioactivity

Measured by its ability to couple reaction catalyzed by PKM2 and LDH, detect the consumption of NADH by pyruvate, the product of phosphate (enol) pyruvate monosodium salt hydrate (PEP). Specific activity is ≥12.13 pmol/min/μg was measured under the conditions described.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag His
  • Accession

    P14618-1 (L61-E480)

  • Gene ID
    5315  [NCBI]
  • Protein Length

    Partial

  • Synonyms

    PKM; Tumor M2-PK; Prev. PKM2; P58; THBP1; Thyroid Hormone-Binding Protein, Cytosolic; OIP3; Epididymis Secretory Protein Li 30; PK3; Pyruvate Kinase Isozymes M1/M2; Pyruvate Kinase Muscle Isozyme; OPA-Interacting Protein 3; Cytosolic Thyroid Hormone-Bindi

  • AA Sequence

    LKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQE

  • Molecular Weight

    Approximately 55 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 1 mM EDTA, trace Imidazole and DTT, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Gupta V, et, al. Human pyruvate kinase M2: a multifunctional protein. Protein Sci. 2010 Nov;19(11):2031-44. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • PKM2 Protein, Human (420a.a, His) Product Image
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  • Experimental Validation Results for PKM2 Protein, Human (420a.a, His)
  • PKM2 Protein, Human (420a.a, His) Product Image