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  4. PKM2 Protein, Human (420 a.a, His)

PKM2 Protein, Human (420 a.a, His)

Cat. No.: HY-P7803
Handling Instructions

PKM2 Protein, Human (420 a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways.

For research use only. We do not sell to patients.

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Description

PKM2 Protein, Human (420 a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways[1].

Background

Pyruvate Kinase M2 (PKM2), a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 is a ubiquitous prototype enzyme present in all tissues during the embryonic stage and is gradually replaced by other isozymic forms in specific tissues during development. Although the primary function of PKM2 is to catabolize glucose, it is involved in many nonglycolytic pathways, influencing the cellular physiology[1].

Biological Activity
Measured by its ability to transfer phosphate from phospho(enol)pyruvic acid monosodium salt hydrate (PEP) to adenosine 5'-diphosphate sodium salt (ADP). The specific activity is >1000 units/mg, as measured under the described conditions.
Species

Human

Source

E. coli

Accession

P14618.4(L61-E480)

Gene ID
Synonyms
rHuPyruvate Kinase M2/PKM2, His; Pyruvate Kinase M2; PKM2; CTHBP; OIP-3; THBP1
AA Sequence

LKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQE

Molecular Weight

Approximately 55 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filter solution of 50 Mm Tris, 1 mM EDTA, trace Imidazole and DTT, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C. It is stable at -20℃ for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

PKM2 Protein, Human (420 a.a, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PKM2 Protein, Human (420 a.a, His)
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HY-P7803
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