PKM - pyruvate kinase M1/2 Gene

Also Known as PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 5315

About PKM

Cytogenetic location: 15q23 Genomic coordinates (GRCh38): 15:72,199,029-72,231,591 (from NCBI)

This gene has 25 transcripts (splice variants), 294 orthologues and 1 paralogue. Ubiquitous expression in brain (RPKM 155.4), esophagus (RPKM 151.3) and 24 other tissues.

Summary

This gene encodes a protein involved in glycolysis. The encoded protein is a Pyruvate Kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid Hormones. This protein has been found to bind Opa protein, a Bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in Bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

PKM Products (18)

mRNA	Protein	Name
XM_047432666.1	XP_047288622.1	pyruvate kinase PKM isoform X8
XM_047432663.1	XP_047288619.1	pyruvate kinase PKM isoform X2
NM_001206797.3	NP_001193726.1	pyruvate kinase PKM isoform d
XM_006720570.2	XP_006720633.1	pyruvate kinase PKM isoform X3
XM_047432665.1	XP_047288621.1	pyruvate kinase PKM isoform X7
XM_047432664.1	XP_047288620.1	pyruvate kinase PKM isoform X6
NM_001206798.3	NP_001193727.1	pyruvate kinase PKM isoform e
NM_182470.4	NP_872270.1	pyruvate kinase PKM isoform b
XM_047432662.1	XP_047288618.1	pyruvate kinase PKM isoform X1
NM_001206799.2	NP_001193728.1	pyruvate kinase PKM isoform f
XM_005254443.2	XP_005254500.1	pyruvate kinase PKM isoform X4
XM_005254445.6	XP_005254502.1	pyruvate kinase PKM isoform X8
NM_182471.4	NP_872271.1	pyruvate kinase PKM isoform b
NM_001316318.2	NP_001303247.1	pyruvate kinase PKM isoform g
NM_001206796.3	NP_001193725.1	pyruvate kinase PKM isoform c
NM_002654.6	NP_002645.3	pyruvate kinase PKM isoform a
NM_001411081.1	NP_001398010.1	pyruvate kinase PKM
XM_011521670.2	XP_011519972.1	pyruvate kinase PKM isoform X5

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
NOT enables histone H3T11 kinase activity	IDA IDA: Inferred from direct assay	22901803	GOA
enables histone H3T11 kinase activity	IDA IDA: Inferred from direct assay	22901803	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	12620389	GOA
enables protein homodimerization activity	IDA IDA: Inferred from direct assay	22306293	GOA
enables protein tyrosine kinase activity	IDA IDA: Inferred from direct assay	22306293	GOA
enables pyruvate kinase activity	IDA IDA: Inferred from direct assay	20005212	GOA
enables transcription coactivator activity	IDA IDA: Inferred from direct assay	22056988	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in glycolytic process	IDA IDA: Inferred from direct assay	20847263	GOA
involved in positive regulation of sprouting angiogenesis	IMP IMP: Inferred from mutant phenotype	27199445	GOA
involved in positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	22056988	GOA
involved in programmed cell death	IDA IDA: Inferred from direct assay	17308100	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in cytoplasm	IDA IDA: Inferred from direct assay	18298799	GOA
is active in nucleus	IDA IDA: Inferred from direct assay	22901803	GOA
located in nucleus	IDA IDA: Inferred from direct assay	17308100	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PKM Protein Structure

PK_C

0
100
200
300
400
500
531 a.a.

Protein Preferred Names	Protein Names
pyruvate kinase PKM	OPA-interacting protein 3 PK, muscle type cytosolic thyroid hormone-binding protein epididymis secretory protein Li 30 pyruvate kinase 2/3 pyruvate kinase isozymes M1/M2 pyruvate kinase muscle isozyme pyruvate kinase, muscle threonine-protein kinase PKM2 thyroid hormone-binding protein 1 thyroid hormone-binding protein, cytosolic tumor M2-PK tyrosine-protein kinase PKM2

PKM Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	PKM	P14618	q9y649_human	Homo sapiens	Q9Y649	Y2H Array	32814053
Intra	PKM	P14618	q9y649_human	Homo sapiens	Q9Y649	Y2H Pooling	32814053
Intra	PKM	P14618	q9y649_human	Homo sapiens	Q9Y649	Validated Y2H	32814053
Intra	PKM	P14618	FGFR3	Homo sapiens	P22607	Y2H Array	32814053
Intra	PKM	P14618	FGFR3	Homo sapiens	P22607	Validated Y2H	32814053
Intra	PKM	P14618	FGFR3	Homo sapiens	P22607	Y2H Pooling	32814053
Intra	PKM	P14618	DAPK1	Homo sapiens	P53355	Anti Bait CoIP	21725354
Intra	PKM	P14618	HTT	Homo sapiens	P42858	Y2H Pooling	32814053
Intra	PKM	P14618	HTT	Homo sapiens	P42858	Validated Y2H	32814053
Intra	PKM	P14618	HTT	Homo sapiens	P42858	Y2H Array	32814053
Intra	PKM	P14618	SPRED1	Homo sapiens	Q7Z699	Y2H Pooling	32814053
Intra	PKM	P14618	SPRED1	Homo sapiens	Q7Z699	Validated Y2H	32814053
Intra	PKM	P14618	SPRED1	Homo sapiens	Q7Z699	Y2H Array	32814053
Intra	PKM	P14618	ARRB2	Homo sapiens	P32121	Anti Tag CoIP	17620599
Intra	PKM	P14618	TINF2	Homo sapiens	Q9BSI4	BiFC	21044950
Intra	PKM	P14618	TINF2	Homo sapiens	Q9BSI4	Pull Down	21044950
Intra	PKM	P14618	ARRB1	Homo sapiens	P49407	Anti Tag CoIP	17620599
Cross	PKM	P14618	polg_hcvco	Hepatitis C virus	Q9WMX2	Anti Bait CoIP	18519040
Cross	PKM	P14618	polg_hcvco	Hepatitis C virus	Q9WMX2	IF	18519040

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant PKM Proteins

Cat. No.	Product Name	Accession	Purity
HY-P7803	PKM2 Protein, Human (420a.a, His)	P14618-1 (L61-E480)	≥ 90%, as determined by reducing SDS-PAGE.
HY-P70599	PKM2 Protein, Human (530a.a, His)	P14618-1 (S2-P531)	≥ 90%, as determined by reducing SDS-PAGE.

PKM Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80870	PKM2 Antibody	WB, IHC-P, ICC/IF, FC	Human, Mouse, Rat
HY-P83644	Phospho-PKM2(Tyr105)Antibody	IHC-P, IHC-F, ICC/IF, FC, ELISA	Human, Rat
HY-P85551	PKM2 Antibody (YA5243)	WB, ICC/IF, IP	Human, Mouse, Monkey
HY-P85552	PKM2 Antibody (YA5244)	WB, ICC/IF	Human, Mouse, Rat, Monkey
HY-P86631	PKM Antibody (YA6323)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse, Rat
HY-P87012	PKM2 Antibody (YA6705)	WB, ICC/IF, IHC-P, FC, IP	Human, Mouse, Rat, Monkey

Related Diseases

Diseases	Alias
Bilirubin Metabolic Disorder	Hyperbilirubinemia Hereditary Hyperbilirubinemia Hyperbilirubinemia, Hereditary Hyperbilirubinaemia
Liver Carcinoma In Situ	Carcinoma In Situ Of Liver And Biliary System Carcinoma In Situ Of Liver, Gallbladder And Bile Ducts
Meningioma, Familial	Meningioma Familial Meningioma Meningioma, Familial, Susceptibility To Meningeal Neoplasm Meningeal Neoplasms Meningiomas Meningioma, Nf2-Related, Somatic Meningioma, Sis-Related Meningothelial Cell Tumor Neoplasm Of The Meninges Primary Meningeal Tumor Familial Multiple Meningioma MNGMA Meningioma, Benign, No Icd-O Subtype Intracranial Meningioma Meningothelial Cell Neoplasm Supratentorial Meningioma Primary Neoplasm Of Spinal Meninges Benign Intracranial Meningioma Benign Meningioma Meningeal Tumours Meningeal Sarcoma Of Unspecified Site Meningothelial Sarcoma Of Unspecified Site
Colorectal Cancer	Colon Cancer Colorectal Carcinoma Colon Carcinoma Colorectal Cancer, Susceptibility To Carcinoma Of Colon CRC Colorectal Cancer With Chromosomal Instability, Somatic Colon Cancer, Somatic Colon Cancer, Susceptibility To Colonic Neoplasms Colorectal Neoplasms Colorectal Cancer, Somatic Colon Cancer, Advanced, Somatic Colonic Carcinoma Colorectal Carcinomas Colon Cancers Colorectal Cancers Cancer, Colorectal, Somatic Cancer, Colon Cancer, Colorectal, Susceptibility To Colorectal Neoplasm Colonic Neoplasm Malignant Tumor Of Colon
Pyruvate Kinase Deficiency Of Red Cells	Pyruvate Kinase Deficiency Pk Deficiency Pyruvate Kinase Deficiency Of Erythrocyte Hemolytic Anemia Due To Red Cell Pyruvate Kinase Deficiency Pyruvate Kinase Deficiency Of Erythrocytes Pkd PKRD Hereditary Non-Spherocytic Hemolytic Anemia Due To Pyruvate Kinase Deficiency Hnsha Pyruvate Kinase-Deficient Hemolytic Anemia Red Cell Pyruvate Kinase Deficiency Deficiency Of Pyruvate Kinase Anemia, Hemolytic, Congenital Nonspherocytic
Glycogen Storage Disease	Glycogenosis Glycogenoses Gsd Storage Disease, Glycogen Gsd - [Glycogen Storage Disease] Glycogen Thesaurismosis Diffuse Glycogenosis Generalised Glycogen Storage Disease Generalised Glycogenosis Generalised Glycogen Storage Disease Of Infants Glycogen Synthase Deficiency
Neonatal Jaundice	Neonatal Hyperbilirubinemia Neonatal Icterus Jaundice Neonatal Jaundice, Neonatal Hyperbilirubinemia, Neonatal
Blood Group Incompatibility
Anterior Dislocation Of Lens
Congenital Nonspherocytic Hemolytic Anemia	Hereditary Nonspherocytic Hemolytic Anemia Hereditary Non-Spherocytic Hemolytic Anemia Anemia, Hemolytic, Congenital Nonspherocytic Congenital Nonspherocytic Hemolytic Anaemia Hereditary Nonspherocytic Hemolytic Anaemia Hnsha
Pigmentation Disease	Pigmentation Disorders Skin Pigmentation Disorder
Renal Cell Carcinoma, Nonpapillary	Renal Cell Carcinoma RCC Nonpapillary Renal Cell Carcinoma Clear Cell Renal Cell Carcinoma Hypernephroma Adenocarcinoma Of Kidney Renal Carcinoma, Chromophobe, Somatic Clear Cell Carcinoma Of Kidney Clear-Cell Metastatic Renal Cell Carcinoma Clear Cell Renal Carcinoma Renal Cell Carcinoma, Somatic Conventional Renal Cell Carcinoma Conventional Renal Cell Carcinoma Renal Clear Cell Carcinoma Ccrcc Hereditary Clear Cell Renal Cell Carcinoma Carcinoma, Renal Cell Renal Cell Carcinoma, Clear Cell, Somatic Renal Cell Carcinoma, Clear Cell Clear Cell Kidney Carcinoma Clear Cell Rcc Cystic-Multilocular Variant Clear Cell Renal Cell Adenocarcinoma Hereditary Clear Cell Renal Cell Adenocarcinoma Common Renal Cell Carcinoma Crcc Renal Cell Carcinoma Non-Papillary Carcinoma Renal Cell Renal Cell Cancer Carcinoma, Renal Cell, Nonpapillary
Pancreatic Cancer	Pancreatic Carcinoma Familial Pancreatic Carcinoma Pancreatic Neoplasm Carcinoma Of Pancreas Pancreatic Carcinoma, Familial Malignant Neoplasm Of Pancreas Pancreatic Acinar Carcinoma Pancreatic Tumor Familial Pancreatic Cancer Neoplasm Of The Pancreas Pancreatic Carcinoma, Somatic Pancreatic Cancer, Somatic Ca Body Of Pancreas Ca Head Of Pancreas Ca Tail Of Pancreas Malignant Neoplasm Of Body Of Pancreas Malignant Neoplasm Of Head Of Pancreas Malignant Neoplasm Of Tail Of Pancreas Pancreas Neoplasm Exocrine Cancer Exocrine Pancreas Carcinoma Hereditary Pancreatic Cancer Hereditary Pancreatic Carcinoma PNCA Cancer Of The Pancreas Pancreatic Cancer, Susceptibility To Carcinoma Of Head Of Pancreas Pancreatic Neoplasms Pancreatic Tumors Cancer, Pancreatic Cancer Of Pancreas Mixed Islet Cell With Exocrine Carcinoma Of Unspecified Site
Kernicterus	Bilirubin Encephalopathy Hyperbilirubinemic Encephalopathy Kernicterus Spectrum Disorder

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Approval
Clinical Phase
Pre-clinical Phase

Approval

Bioactive Molecules (2)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-B0404A	Benserazide hydrochloride	Inhibitor	99.68%	Yes
HY-B0404AR	Benserazide hydrochloride (Standard)	Inhibitor	99.89%	Unkown

Clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Phase
HY-P1284	ZIP	Inhibitor	98.56%	Phase 4
HY-155785	MTP		/	Phase 4
HY-P1284A	ZIP TFA	Inhibitor	/	Phase 4

Pre-clinical Phase

Bioactive Molecules (59)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-N0822	Shikonin	Inhibitor	99.80%	Unkown
HY-18657	TEPP-46	Activator	99.93%	Unkown
HY-103617	PKM2-IN-1	Inhibitor	99.79%	Unkown
HY-19330	DASA-58	Activator	99.57%	Unkown
HY-126585	SAICAR		99.57%	Unkown
HY-162080	METTL1-WDR4-IN-1	Inhibitor	99.61%	Unkown
HY-N7108	7-Hydroxyflavone		99.89%	Unkown
HY-157913	PKM2-IN-6		99.95%	Unkown
HY-147368	PKM2 activator 2	Activator	99.38%	Unkown
HY-167832	PT109		99.20%	Unkown
HY-N4185	Licoflavone A		99.95%	Unkown
HY-121275	Benserazide	Inhibitor	98.43%	Yes
HY-139667	PKM2-IN-3	Inhibitor	99.44%	Unkown
HY-N2552	Vitamin K5 hydrochloride		99.25%	Unkown
HY-D1456	TEPC466		99.43%	Unkown
HY-N3011	Iridin		99.72%	Unkown
HY-N7064	Iminostilbene	Inhibitor	99.66%	Unkown
HY-148324	PKM2 activator 5	Activator	96.03%	Unkown
HY-148615	NPD10084	Inhibitor	99.69%	Unkown
HY-155850	CIAC001	Inhibitor	99.70%	Unkown
HY-W587878	Thujopsene		86.31%	Unkown
HY-P1399A	Pep2m, myristoylated TFA	Inhibitor	99.77%	Unkown
HY-126585S	SAICAR-d3		96.57%	Unkown
HY-132215	PF-06284674	Activator	99.69%	Unkown
HY-156375	PKM2 activator 6		/	Unkown
HY-162080A	METTL1-WDR4-IN-1 TFA	Inhibitor	99.61%	Unkown
HY-179371	LIQ1	Inhibitor	97.40%	Unkown
HY-D1876	ZY-2		95.77%	Unkown
HY-RS10602	PKM Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-119874	Alkannin	Inhibitor	/	Unkown
HY-147397	PKM2 activator 3	Activator	/	Unkown
HY-149275	PKM2/PDK1-IN-1		/	Unkown
HY-155216	PKM2 activator 10	Activator	/	Unkown
HY-155668	HMG-CoA Reductase-IN-1	Inhibitor	/	Unkown
HY-155849	PKM2-IN-4	Inhibitor	/	Unkown
HY-161519	PKM2 activator 7	Activator	/	Unkown
HY-168882	PKM2 modulator 1		/	Unkown
HY-168883	PKM2 modulator 2		/	Unkown
HY-169567	PKM2-IN-9	Inhibitor	/	Unkown
HY-170577	PKM2-IN-8		/	Unkown
HY-172881	PKM2-IN-10	Inhibitor	/	Unkown
HY-178441	PKM2-IN-11		/	Unkown
HY-178748	PKM2-IN-12		/	Unkown
HY-180523	PKM2-IN-13		/	Unkown
HY-180795	PKM2 activator 11		/	Unkown
HY-182037	Multi-target kinase-IN-9	Inhibitor	/	Unkown
HY-183765	PARP1/PKM2-IN-1	Inhibitor	/	Unkown
HY-B0404AS	Benserazide-d3 hydrochloride	Inhibitor	/	Unkown
HY-B1814	Vitamin K5		/	Unkown
HY-D1877	ZY-1		98.91%	Unkown
HY-P1399	Pep2m, myristoylated	Inhibitor	/	Unkown
HY-RS10603	Pkm Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS10604	Pkm Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-W753956	Iminostilbene-d10		/	Unkown
HY-148313	PKM2 activator 4	Activator	/	Unkown
HY-162071	PKM2-IN-5	Inhibitor	/	Unkown
HY-168995	BAR-072	Inhibitor	/	Unkown
HY-169883	PKM2-IN-7	Inhibitor	/	Unkown
HY-180796	PKM2 agonist-1	Agonist	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Bos taurus	PKM	VGNC	VGNC:55132
Rattus norvegicus	PKM	RGD	RGD:3337
Mus musculus	PKM	MGD	MGI:97591
Others	PKM	NCBI