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Cat. No.: HY-P12310

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Ac-PKM-AMC

Ac-PKM-AMC is a fluorescent tripeptide substrate for ClpP1P2 peptidase (excitation and emission: 380/460 nm). Ac-PKM-AMC is used to measure Mtb ClpP1P2 peptidase activity.

For research use only. We do not sell to patients.

CAS No.: 1431362-34-3
Formula: C₂₈H₃₉N₅O₆S
Molecular Weight:573.70

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Ac-PKM-AMC Chemical Structure

CAS No.: 1431362-34-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

Ac-PKM-AMC is a fluorescent tripeptide substrate for ClpP1P2 peptidase (excitation and emission: 380/460 nm). Ac-PKM-AMC is used to measure Mtb ClpP1P2 peptidase activity^[1]^[2]^[3].

In Vitro

Ac-PKM-AMC (100 μM) serves as the fluorogenic substrate for measuring Mtb ClpP1P2 peptidase activity, with cleavage monitored by fluorescence at 380/460 nm^[1].
Ac-PKM-AMC (10 μM; at least 1 h) is the substrate used in the ClpP1P2 peptidase degradation assay to determine IC₅₀ values of inhibitors^[1].
Ac-PKM-AMC hydrolysis by Mtb ClpP1P2 is dramatically stimulated by Bz-LL or Bz-Nva-Ile in the presence of ClpX or ClpC1, with half-maximal activation requiring an order of magnitude lower activator concentration when either ATPase is present^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1431362-34-3
Molecular Weight 573.70
Formula C₂₈H₃₉N₅O₆S
SMILES

CC(C1=CC=C2NC([C@H](CCSC)NC([C@H](CCCCN)NC([C@H]3N(C(C)=O)CCC3)=O)=O)=O)=CC(OC1=C2)=O
Sequence

Ac-Pro-Lys-Met-AMC
Sequence Shortening

Ac-PKM-AMC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Xiao G, et al. Identification of a phenyl ester covalent inhibitor of caseinolytic protease and analysis of the ClpP1P2 inhibition in mycobacteria. mLife. 2025 Apr;4(2):155-168. [Content Brief]

[2]. Li M, et al. Structure and Functional Properties of the Active Form of the Proteolytic Complex, ClpP1P2, from Mycobacterium tuberculosis. The Journal of biological chemistry. 2016 Apr 01;291(14):7465-76.

[3]. Akopian T, et al. Cleavage Specificity of Mycobacterium tuberculosis ClpP1P2 Protease and Identification of Novel Peptide Substrates and Boronate Inhibitors with Anti-bacterial Activity. The Journal of biological chemistry. 2015 Apr 24;290(17):11008-20.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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