1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. SAICAR

SAICAR 

Cat. No.: HY-126585 Purity: 99.66% ee.: 100%
Handling Instructions

SAICAR is an intermediate of de novo purine nucleotide biosynthesis, activates pyruvate kinase isoform M2 (PKM2) in an isozyme-selective manner, with an EC50 of 0.3 mM. SAICAR stimulates PKM2 and promotes cancer cell survival in glucose-limited conditions.

For research use only. We do not sell to patients.

SAICAR Chemical Structure

SAICAR Chemical Structure

CAS No. : 3031-95-6

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500 μg USD 200 In-stock
Estimated Time of Arrival: December 31
1 mg USD 340 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

SAICAR is an intermediate of de novo purine nucleotide biosynthesis, activates pyruvate kinase isoform M2 (PKM2) in an isozyme-selective manner, with an EC50 of 0.3 mM. SAICAR stimulates PKM2 and promotes cancer cell survival in glucose-limited conditions[1][2].

IC50 & Target[1]

Human Endogenous Metabolite

 

In Vitro

SAICAR accumulation induces nuclear localization of PKM2. PKM2-SAICAR phosphorylates and activates Erk1/2, which in turn sensitizes PKM2 for SAICAR binding through phosphorylation. Additionally, PKM2-SAICAR was necessary to induce sustained Erk1/2 activation and mitogen-induced cell proliferation. SAICAR-PKM2 interaction is necessary and sufficient to induce H3 T11 and Erk1/2 phosphorylation[2].
Upon glucose starvation, cellular SAICAR concentration increases in an oscillatory manner and stimulates PKM2 activity in cancer cells. The SAICAR-PKM2 interaction also promotes cancer cell survival in glucose-limited conditions. In glucose-limited conditions, cells with higher SAICAR concentrations (adsl-kd cells or cells overexpressing PAICS) survive better while paics-kd cells died earlier than control-kd cells. SAICAR promotes cancer cell survival in glucose-limited conditions[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

454.28

Formula

C₁₃H₁₉N₄O₁₂P

CAS No.
SMILES

O=C(O)C[[email protected]@H](C(O)=O)NC(C1=C(N)N([[email protected]]2[[email protected]@H]([[email protected]@H]([[email protected]@H](COP(O)(O)=O)O2)O)O)C=N1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 220 mg/mL (484.28 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2013 mL 11.0064 mL 22.0129 mL
5 mM 0.4403 mL 2.2013 mL 4.4026 mL
10 mM 0.2201 mL 1.1006 mL 2.2013 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 5.5 mg/mL (12.11 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 5.5 mg/mL (12.11 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 5.5 mg/mL (12.11 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.66% ee.: 100%

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Keywords:

SAICAREndogenous Metabolitepurinenucleotidebiosynthesismetaboliteglucose-limitedcancercellproliferationSAICAR-PKM2interactionsurvivalInhibitorinhibitorinhibit

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Product Name:
SAICAR
Cat. No.:
HY-126585
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