PLD4 Protein, Human (HEK293, His)

PLD4 Protein, a phospholipase, plays a crucial role in immune responses and inflammation. Dysregulation of PLD4 Protein has been associated with autoimmune disorders and inflammatory diseases. Targeting PLD4 Protein may provide potential therapeutic interventions in these conditions by modulating immune responses, suppressing inflammation, and managing related disorders. PLD4 Protein, Human (HEK293, His) is the recombinant human-derived PLD4 protein, expressed by HEK293, with C-His labeled tag. The total length of PLD4 Protein, Human (HEK293, His) is 455 a.a., with molecular weight of 68-75 kDa.

PLD4 Protein, a 5'->3' DNA exonuclease, is responsible for digesting single-stranded DNA (ssDNA). Its role extends to regulating inflammatory cytokine responses by degrading nucleic acids, thereby decreasing the concentration of ssDNA capable of stimulating TLR9, a nucleotide-sensing receptor. Additionally, PLD4 Protein is involved in the phagocytosis of activated microglia.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-His

Q96BZ4 (W52-G506)

122618  [NCBI]

68-75 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM MES, 100 mM NaCl, pH 6.0. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.