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  4. PRG3 Protein, Human (HEK293, His)

PRG3 Protein, Human (HEK293, His)

Cat. No.: HY-P71233
Handling Instructions

PRG3 Protein is a stress-resistant extracellular matrix structural component. PRG3 Protein can stimulate granulocyte activation, histamine synthesis and regulation of gene expression. PRG3 Protein, Human (HEK293, His) is the recombinant human-derived PRG3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRG3 Protein, Human (HEK293, His) is 208 a.a., with molecular weight of ~27.0 kDa.

For research use only. We do not sell to patients.

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Description

PRG3 Protein is a stress-resistant extracellular matrix structural component. PRG3 Protein can stimulate granulocyte activation, histamine synthesis and regulation of gene expression. PRG3 Protein, Human (HEK293, His) is the recombinant human-derived PRG3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRG3 Protein, Human (HEK293, His) is 208 a.a., with molecular weight of ~27.0 kDa.
Background

Proteoglycan 3 is a stress-resistant extracellular matrix structural component and has similar cytotoxic and costimulatory activities to PRG2/MBP. PRG3 stimulates superoxide production and IL8 release of basophils in vitro, as well as histamine and leukotriene C4 release[1][2][3].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

AAI13412.1 (L18-F225)
Gene ID
Molecular Construction
N-term
PRG3 (L18-F225)
Accession # AAI13412.1
6*His
C-term
Synonyms
Proteoglycan 3; Eosinophil Major Basic Protein Homolog; Prepro-Major Basic Protein Homolog; Prepro-MBPH; PRG3; MBPH
AA Sequence

LHLENDAPHLESLETQADLGQDLDSSKEQERDLALTEEVIQAEGEEVKASACQDNFEDEEAMESDPAALDKDFQCPREEDIVEVQGSPRCKTCRYLLVRTPKTFAEAQNVCSRCYGGNLVSIHDFNFNYRIQCCTSTVNQAQVWIGGNLRGWFLWKRFCWTDGSHWNFAYWSPGQPGNGQGSCVALCTKGGYWRRAQCDKQLPFVCSF

Molecular Weight

Approximately 27.0 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

PRG3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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