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  5. Prostasin/PRSS8 Protein, Mouse (sf9, His)

Prostasin/PRSS8 Protein, Mouse (sf9, His)

Cat. No.: HY-P77154
Handling Instructions

Prostasin/PRSS8 Protein, featuring trypsin-like cleavage specificity, crucially activates the epithelial sodium channel (ENaC) by cleaving gamma subunits (SCNN1G) and modulating ion transport. Structurally, it forms a disulfide bond-linked heterodimer with light and heavy chains, highlighting its intricate role in cellular responses. Prostasin/PRSS8 Protein, Mouse (sf9, His) is the recombinant mouse-derived Prostasin/PRSS8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Prostasin/PRSS8 Protein, Mouse (sf9, His) is 260 a.a., with molecular weight of ~35 kDa.

For research use only. We do not sell to patients.

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Description

Prostasin/PRSS8 Protein, featuring trypsin-like cleavage specificity, crucially activates the epithelial sodium channel (ENaC) by cleaving gamma subunits (SCNN1G) and modulating ion transport. Structurally, it forms a disulfide bond-linked heterodimer with light and heavy chains, highlighting its intricate role in cellular responses. Prostasin/PRSS8 Protein, Mouse (sf9, His) is the recombinant mouse-derived Prostasin/PRSS8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Prostasin/PRSS8 Protein, Mouse (sf9, His) is 260 a.a., with molecular weight of ~35 kDa.
Background

Prostasin/PRSS8, characterized by a trypsin-like cleavage specificity with a preference for poly-basic substrates, plays a crucial role in stimulating the activity of the epithelial sodium channel (ENaC). Its activation involves the cleavage of the gamma subunits (SCNN1G) to modulate ion transport processes. Structurally, Prostasin/PRSS8 forms a heterodimer comprising light and heavy chains, united by a disulfide bond, underscoring its functional complexity in mediating cellular responses.
Species

Mouse
Source

Sf9 insect cells
Tag

C-His
Accession

EDL17608 (A30-Q289)
Gene ID

76560  [NCBI]
Molecular Construction
N-term
Prostasin (A30-Q289)
Accession # EDL17608
His
C-term
Synonyms
Channel-activating protease 1; CAP1; Serine protease 8
Molecular Weight

Approximately 35 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Prostasin/PRSS8 Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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