PSAP/Prosaposin Protein, Mouse (HEK293, His)
Based on 1 publication(s) in Google Scholar
PSAP/Prosaposin protein is a myelin trophic factor and neurotrophic factor that acts through GPR37 and GPR37L1 receptors.This triggers ligand-mediated internalization, initiating ERK phosphorylation signaling.PSAP/Prosaposin Protein, Mouse (HEK293, His) is the recombinant mouse-derived PSAP/Prosaposin protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PSAP/Prosaposin protein is a myelin trophic factor and neurotrophic factor that acts through GPR37 and GPR37L1 receptors.This triggers ligand-mediated internalization, initiating ERK phosphorylation signaling.PSAP/Prosaposin Protein, Mouse (HEK293, His) is the recombinant mouse-derived PSAP/Prosaposin protein, expressed by HEK293 , with C-His labeled tag.
Background
The PSAP/Prosaposin protein serves as a myelinotrophic and neurotrophic factor, exerting its effects through G-protein-coupled receptors, GPR37 and GPR37L1. This interaction triggers ligand-mediated internalization, subsequently initiating ERK phosphorylation signaling. Furthermore, the protein exhibits regulatory prowess in sphingolipid metabolism, as both Saposin-A and Saposin-C facilitate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Notably, Saposin-C's mode of action involves forming an activated complex with the enzyme and acidic lipid, rather than solubilizing the substrate, highlighting its nuanced role in cellular processes.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
Q61207/NP_035309.3 (S17-N557)
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Molecular Construction
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N-term
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PSAP (S17-N557)
Accession # Q61207/NP_035309.3 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
PSAP; Saposin-A; Prev. GLBA; Saposin-C; Prev. SAP1; Saposin-D; Prev. SAP2; Variant Gaucher Disease And Variant Metachromatic Leukodystrophy; Sphingolipid Activator Protein-1; Alternative Protein PSAP; Sphingolipid Activator Protein-2; PARK24; Proactivator
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AA Sequence
SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN
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Predicted Molecular Mass
60.9 kDa
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Molecular Weight
Approximately 61-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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