PSAP/Prosaposin Protein, Mouse (HEK293, His)

PSAP/Prosaposin Protein, a myelinotrophic and neurotrophic factor, acts through GPR37 and GPR37L1 receptors. This triggers ligand-mediated internalization, initiating ERK phosphorylation signaling. Additionally, PSAP/Prosaposin regulates sphingolipid metabolism, with Saposin-A and Saposin-C facilitating glucosylceramide and galactosylceramide hydrolysis. Notably, Saposin-C forms an activated complex with the enzyme and acidic lipid, emphasizing its nuanced role in cellular processes. PSAP/Prosaposin Protein, Mouse (HEK293, His) is the recombinant mouse-derived PSAP/Prosaposin protein, expressed by HEK293, with C-His labeled tag. The total length of PSAP/Prosaposin Protein, Mouse (HEK293, His) is 541 a.a., with molecular weight of ~61.2 KDa.

The PSAP/Prosaposin protein serves as a myelinotrophic and neurotrophic factor, exerting its effects through G-protein-coupled receptors, GPR37 and GPR37L1. This interaction triggers ligand-mediated internalization, subsequently initiating ERK phosphorylation signaling. Furthermore, the protein exhibits regulatory prowess in sphingolipid metabolism, as both Saposin-A and Saposin-C facilitate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Notably, Saposin-C's mode of action involves forming an activated complex with the enzyme and acidic lipid, rather than solubilizing the substrate, highlighting its nuanced role in cellular processes.

Mouse

HEK293

C-His

Q61207/NP_035309.3 (S17-N557)

19156  [NCBI]

SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Approximately 61.2 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.