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  5. PSG1 Protein, Human (HEK293, His)

PSG1 Protein, Human (HEK293, His)

Cat. No.: HY-P72486
SDS COA Handling Instructions

PSG1 Protein is extremely vital to development and health of a fetus. PSG1 Protein is important in inducing, enhancing or inhibiting an immune response. PSG1 Protein, Human (HEK293, His) is the recombinant human-derived PSG1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PSG1 Protein, Human (HEK293, His) is 385 a.a., with molecular weight of ~68 kDa.

For research use only. We do not sell to patients.

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10 μg $180 In-stock
50 μg $500 In-stock
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  • Biological Activity

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Description

PSG1 Protein is extremely vital to development and health of a fetus. PSG1 Protein is important in inducing, enhancing or inhibiting an immune response[1][2]. PSG1 Protein, Human (HEK293, His) is the recombinant human-derived PSG1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PSG1 Protein, Human (HEK293, His) is 385 a.a., with molecular weight of ~68 kDa.

Background

PSG1 Protein is a secreted glycoprotein of the human PSG family[3]. PSG1 Protein is the most abundant PSG in maternal serum, as a possible inducer of proangiogenic growth factors known to play an important role in establishment of the vasculature at the maternal-fetal interface[4]. PSG1 Protein functions primarily as an immune-modulator to protect the growing fetus[5].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P11464/NP_001171754.1 (Q35-P419)

Gene ID
Molecular Construction
N-term
PSG1 (Q35-P419)
Accession # P11464/NP_001171754.1
6*His
C-term
Synonyms
Pregnancy-specific beta-1-glycoprotein 1; PSBG-1; FL-NCA-1/2; PSG95; CD66f; B1G1; PSGGA
AA Sequence

QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEVLYLSCSADSNPPAQYSWTINEKFQLPGQKLFIRHITTKHSGLYVCSVRNSATGKESSKSMTVEVSDWTVP

Molecular Weight

Approximately 68 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PSG1 Protein, Human (HEK293, His)
Cat. No.:
HY-P72486
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