PSP Protein, Human (His)
Based on 1 Customer Validation
PSP protein catalyzes the final irreversible step in carbohydrate biosynthesis of L-serine through dephosphorylation of O-phospho-L-serine. PSP Protein, Human (His) is the recombinant human-derived PSP protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PSP protein catalyzes the final irreversible step in carbohydrate biosynthesis of L-serine through dephosphorylation of O-phospho-L-serine. PSP Protein, Human (His) is the recombinant human-derived PSP protein, expressed by E. coli , with C-6*His labeled tag.
Phosphoserine phosphatase (PSP) is responsible for catalyzing the final irreversible step in the biosynthesis of L-serine from carbohydrates, involving the dephosphorylation of O-phospho-L-serine to L-serine. This enzymatic activity is pivotal for various cellular processes, as L-serine serves as a precursor for protein synthesis, the generation of other amino acids, nucleotide metabolism, and glutathione synthesis. Additionally, L-serine can undergo racemization to form D-serine, which functions as a neuromodulator. PSP's role in regulating the availability of L-serine underscores its significance in diverse metabolic pathways and cellular functions. There is also a potential involvement in the dephosphorylation of O-phospho-D-serine, although this aspect remains to be confirmed.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P78330 (M1-E225)
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Molecular Construction
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N-term
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PSP (M1-E225)
Accession # P78330 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
PSPH; L-3-Phosphoserine Phosphatase; Prev. PSP; PSPHD; O-Phosphoserine Phosphohydrolase; Phosphoserine Phosphatase; PSPase
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AA Sequence
MVSHSELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQPSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELEE
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Predicted Molecular Mass
26.1 kDa
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Molecular Weight
Approximately 25-30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 4 M Urea, 5 mM EDTA, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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