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  3. Isomerases (EC 5)
  4. PTGDS Protein, Human (HEK293, His)

PTGDS Protein, Human (HEK293, His)

Cat. No.: HY-P71242
COA Handling Instructions

The PTGDS protein catalyzes the conversion of PGH2 to PGD2, a prostaglandin that affects smooth muscle activity and platelet aggregation. It plays a role in central nervous system functions such as sedation, sleep, and allodynia. PTGDS Protein, Human (HEK293, His) is the recombinant human-derived PTGDS protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PTGDS Protein, Human (HEK293, His) is 168 a.a., with molecular weight of 20-29 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The PTGDS protein catalyzes the conversion of PGH2 to PGD2, a prostaglandin that affects smooth muscle activity and platelet aggregation. It plays a role in central nervous system functions such as sedation, sleep, and allodynia. PTGDS Protein, Human (HEK293, His) is the recombinant human-derived PTGDS protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PTGDS Protein, Human (HEK293, His) is 168 a.a., with molecular weight of 20-29 kDa.

Background

The PTGDS protein serves a multifaceted role in various physiological processes. It catalyzes the conversion of PGH2 to PGD2, a prostaglandin with implications in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Beyond its involvement in these vascular functions, PTGDS plays a crucial role in diverse central nervous system (CNS) activities, including sedation, NREM sleep, and PGE2-induced allodynia, and may exhibit an anti-apoptotic function in oligodendrocytes. Notably, PTGDS has a broad substrate-binding capacity, interacting with small non-substrate lipophilic molecules such as biliverdin, bilirubin, retinal, retinoic acid, and thyroid hormone. This suggests its potential role as a scavenger for harmful hydrophobic molecules and as a transporter for secretory retinoids and thyroid hormones. Additionally, PTGDS is implicated in the development and maintenance of various blood barriers, including the blood-brain, blood-retina, blood-aqueous humor, and blood-testis barriers, indicating its significance in barrier function. Furthermore, it plays essential roles in the maturation and maintenance of the central nervous system and the male reproductive system, as well as participates in PLA2G3-dependent maturation of mast cells, further emphasizing its intricate involvement in diverse biological processes.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P41222 (A23-Q190)

Gene ID
Molecular Construction
N-term
PTGDS (A23-Q190)
Accession # P41222
6*His
C-term
Synonyms
Prostaglandin-H2 D-Isomerase; Beta-Trace Protein; Cerebrin-28; Glutathione-Independent PGD Synthase; Lipocalin-Type Prostaglandin-D Synthase; Prostaglandin-D2 Synthase; PGD2 Synthase; PGDS; PGDS2; PTGDS; PDS
AA Sequence

APEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKKAALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSVVETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQTDKCMTEQ

Molecular Weight

20-29 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10%Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PTGDS Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PTGDS Protein, Human (HEK293, His)
Cat. No.:
HY-P71242
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