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PTK7 Protein, Human (HEK293, His)

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The PTK7 protein is an inactive tyrosine kinase that plays a key role in both non-canonical and canonical Wnt signaling pathways. Effects include cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 Protein, Human (HEK293, His-Avi) is the recombinant human-derived PTK7 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • PTK7 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The PTK7 protein is an inactive tyrosine kinase that plays a key role in both non-canonical and canonical Wnt signaling pathways. Effects include cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 Protein, Human (HEK293, His-Avi) is the recombinant human-derived PTK7 protein, expressed by HEK293 , with C-His labeled tag.

Background

The PTK7 protein, an inactive tyrosine kinase, plays a significant role in the Wnt signaling pathway, being a component of both the non-canonical (Wnt/planar cell polarity signaling) and canonical Wnt signaling pathways. Its involvement spans diverse cellular processes, including cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 also contributes to critical developmental events, such as embryogenesis, epithelial tissue organization, and angiogenesis. Notably, it interacts with CTNNB1, underlining its participation in Wnt pathway regulation and its impact on cellular functions with broader implications in both normal and pathological contexts.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Immobilized Human PTK7, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 2-3 ng/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q13308-1 (A31-T704)

  • Gene ID
    5754  [NCBI]
  • Molecular Construction
    • N-term
    • PTK7 (A31-T704)
      Accession # Q13308-1
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PTK7; PTK7 Protein Tyrosine Kinase 7; Protein Tyrosine Kinase 7 (Inactive); Tyrosine-Protein Kinase-Like 7; CCK4; CCK-4; Colon Carcinoma Kinase 4; Protein Tyrosine Kinase 7; Inactive Tyrosine-Protein Kinase 7; Protein-Tyrosine Kinase 7; Pseudo Tyrosine Ki

  • AA Sequence

    AIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPAGPEHSGLYSCCAHSAFGQACSSQNFTLSIADESFARVVLAPQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLANIAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQDSQLEEGKPGYLDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYRCMSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT

  • Predicted Molecular Mass

    75.7 kDa

  • Molecular Weight

    Approximately 80-125 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5-8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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