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PTK7 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Ptk7 Protein is a cell surface receptor that plays a crucial role in regulating cell migration and tissue development. Dysregulation of Ptk7 Protein has been associated with various diseases, including cancer and developmental disorders. Targeting Ptk7 Protein may offer potential therapeutic interventions in these conditions by modulating cell migration, inhibiting tumor growth, and promoting normal tissue development. PTK7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PTK7 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for PTK7 Protein, Mouse (HEK293, His)
  • PTK7 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

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Biological Activity

Description

Ptk7 Protein is a cell surface receptor that plays a crucial role in regulating cell migration and tissue development. Dysregulation of Ptk7 Protein has been associated with various diseases, including cancer and developmental disorders. Targeting Ptk7 Protein may offer potential therapeutic interventions in these conditions by modulating cell migration, inhibiting tumor growth, and promoting normal tissue development. PTK7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

Ptk7 Protein is an inactive tyrosine kinase that plays a crucial role in the Wnt signaling pathway, specifically in both the non-canonical (Wnt/planar cell polarity signaling) and canonical Wnt signaling pathways. It is involved in various cellular processes, including cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. Ptk7 Protein also contributes to embryogenesis, epithelial tissue organization, and angiogenesis. Additionally, it interacts with CTNNB1, further highlighting its significance in Wnt signaling.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Recombinant Mouse PTK7/CCK4 binds Biotinylated Recombinant Mouse Wnt-3a. The ED50 for this effect is 7.183 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q8BKG3 (A23-T696)

  • Gene ID
    71461  [NCBI]
  • Molecular Construction
    • N-term
    • PTK7 (A23-T696)
      Accession # Q8BKG3
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PTK7; PTK7 Protein Tyrosine Kinase 7; Protein Tyrosine Kinase 7 (Inactive); Tyrosine-Protein Kinase-Like 7; CCK4; CCK-4; Colon Carcinoma Kinase 4; Protein Tyrosine Kinase 7; Inactive Tyrosine-Protein Kinase 7; Protein-Tyrosine Kinase 7; Pseudo Tyrosine Ki

  • AA Sequence

    AIVFIKEPSSQDALQGRRALLRCEVEAPDPVHVYWLLNGVPVQDTERRFAQGSSLSFAAVDRLQDSGAFQCVARDNVTGEEVRSTNASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDDQSTHTVSSRERNLTLRPASPEHSGLYSCCAHNAFGQACSSQNFTLSVADESFARVVLAPQDVVVARNEEAMFHCQFSAQPPPSLQWVFEDETPITNRSRPPHLRRAVVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPIVLEATLHLAEIEDMLPFEPRVFIAGDEERVTCPAPQGLPTPSVWWEHAGVPLPAHGRVHQKGLELVFVTIAESDTGVYTCHASNLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQATPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTLYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVDKPVMEDSEGPGSPPPYKMIQT

  • Molecular Weight

    Approximately 100-115 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Recombinant Mouse PTK7/CCK4 binds Biotinylated Recombinant Mouse Wnt-3a. The ED50 for this effect is 7.183 μg/mL.

  • PTK7 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PTK7 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for PTK7 Protein, Mouse (HEK293, His)
  • PTK7 Protein, Mouse (HEK293, His) Product Image