PYHIN1 Protein, Human (His, StrepⅡ, Flag)

PYHIN1 Protein, a major mediator of IFN's tumor suppressor activity in breast cancer cells, facilitates the ubiquitination and degradation of MDM2, leading to p53/TP53 stabilization. It also promotes the ubiquitination and degradation of HDAC1, enhancing maspin expression and impairing the invasive activity of cancer cells. PYHIN1 interacts with MDM2 in these regulatory processes. PYHIN1 Protein, Human (His, Strep) is the recombinant human-derived PYHIN1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.

PYHIN1 Protein emerges as a key mediator in breast cancer cells, playing a pivotal role in amplifying the tumor suppressor activity of IFN. It facilitates the ubiquitination and subsequent degradation of MDM2, resulting in the stabilization of p53/TP53. Additionally, PYHIN1 promotes the ubiquitination and subsequent degradation of HDAC1, leading to increased maspin expression and a reduction in the invasive activity of cancer cells. Its interaction with MDM2 underscores its intricate involvement in regulatory pathways critical for cancer cell behavior.

Human

E. coli

C-Flag;N-StrepⅡ;N-6*His

Q6K0P9 (M1-P492)

149628

Full Length

MANNYKKIVLLKGLEVINDYHFRIVKSLLSNDLKLNPKMKEEYDKIQIADLMEEKFPGDAGLGKLIEFFKEIPTLGDLAETLKREKLKVANKIESIPVKGIIPSKKTKQKEVYPATPACTPSNRLTAKGAEETLGPQKRKKPSEEETGTKRSKMSKEQTRPSCSAGASTSTAMGRSPPPQTSSSAPPNTSSTESLKPLANRHATASKNIFREDPIIAMVLNATKVFKYESSENEQRRMFHATVATQTQFFHVKVLNINLKRKFIKKRIIIISNYSKRNSLLEVNEASSVSEAGPDQTFEVPKDIIRRAKKIPKINILHKQTSGYIVYGLFMLHTKIVNRKTTIYEIQDKTGSMAVVGKGECHNIPCEKGDKLRLFCFRLRKRENMSKLMSEMHSFIQIQKNTNQRSHDSRSMALPQEQSQHPKPSEASTTLPESHLKTPQMPPTTPSSSSFTKKDETHPGAQSSPANFRITSPTVAPPLSSDTSTNRHPAVP

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.