RPLP1 Protein, Human (sf9, His)

The RPLP1 protein plays a critical role in the fundamental cellular process of elongation of protein synthesis. It binds to RPLP2 and forms a heterodimer on the ribosome side stalk, positioning RPLP1 as a component of the ribosomal machinery. RPLP1 Protein, Human (sf9, His) is the recombinant human-derived RPLP1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The RPLP1 protein plays a critical role in the fundamental cellular process of elongation of protein synthesis. It binds to RPLP2 and forms a heterodimer on the ribosome side stalk, positioning RPLP1 as a component of the ribosomal machinery. RPLP1 Protein, Human (sf9, His) is the recombinant human-derived RPLP1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

RPLP1 protein assumes a significant role in the elongation step of protein synthesis, a fundamental process in cellular protein production. Operating in conjunction with RPLP2, it forms a heterodimer at the lateral ribosomal stalk of the large ribosomal subunit. This structural arrangement positions RPLP1 as an integral component of the ribosomal machinery crucial for facilitating the elongation phase of protein synthesis, underscoring its involvement in the precise coordination of ribosomal functions during this essential cellular process. The heterodimeric association with RPLP2 further emphasizes the collaborative nature of these ribosomal proteins in orchestrating the intricate steps of protein elongation within the ribosome.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • His
    • RPLP1 (M1-D114)
      Accession # P05386-1/NP_000994.1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    RPLP1; P1; Ribosomal Protein Lateral Stalk Subunit P1; Ribosomal Protein, Large, P1; Large Ribosomal Subunit Protein P1; Acidic Ribosomal Phosphoprotein P1; 60S Acidic Ribosomal Protein P1; RPP1; LP1; RRP1

  • AA Sequence

    MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD

  • Predicted Molecular Mass

    13.8 kDa

  • Molecular Weight

    Approximately 18-25 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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