RPLP1 Protein, Human (sf9, His)
The RPLP1 protein plays a critical role in the fundamental cellular process of elongation of protein synthesis. It binds to RPLP2 and forms a heterodimer on the ribosome side stalk, positioning RPLP1 as a component of the ribosomal machinery. RPLP1 Protein, Human (sf9, His) is the recombinant human-derived RPLP1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
The RPLP1 protein plays a critical role in the fundamental cellular process of elongation of protein synthesis. It binds to RPLP2 and forms a heterodimer on the ribosome side stalk, positioning RPLP1 as a component of the ribosomal machinery. RPLP1 Protein, Human (sf9, His) is the recombinant human-derived RPLP1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
RPLP1 protein assumes a significant role in the elongation step of protein synthesis, a fundamental process in cellular protein production. Operating in conjunction with RPLP2, it forms a heterodimer at the lateral ribosomal stalk of the large ribosomal subunit. This structural arrangement positions RPLP1 as an integral component of the ribosomal machinery crucial for facilitating the elongation phase of protein synthesis, underscoring its involvement in the precise coordination of ribosomal functions during this essential cellular process. The heterodimeric association with RPLP2 further emphasizes the collaborative nature of these ribosomal proteins in orchestrating the intricate steps of protein elongation within the ribosome.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
P05386-1/NP_000994.1 (M1-D114)
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Molecular Construction
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N-term
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His
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RPLP1 (M1-D114)
Accession # P05386-1/NP_000994.1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
RPLP1; P1; Ribosomal Protein Lateral Stalk Subunit P1; Ribosomal Protein, Large, P1; Large Ribosomal Subunit Protein P1; Acidic Ribosomal Phosphoprotein P1; 60S Acidic Ribosomal Protein P1; RPP1; LP1; RRP1
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AA Sequence
MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD
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Predicted Molecular Mass
13.8 kDa
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Molecular Weight
Approximately 18-25 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)