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SARS-CoV-2 3CLpro/3C-like protease Protein (His)

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Based on 1 Customer Validation

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus.

For research use only. We do not sell to patients.
  • Species: Virus
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for SARS-CoV-2 3CLpro/3C-like protease Protein (His)
  • SARS-CoV-2 3CLpro/3C-like protease Protein (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus[1].

Background

The 3C protease has been regarded as a valid target due to its structural similarity among different picornaviruses and minimal sequence similarity with host proteins; therefore, the development of potent inhibitors against the 3C protease as an antiviral drug is ongoing. Duck hepatitis A virus (DHAV) belongs to the Picornavidea family and is a major threat to the poultry industry[1].

Verified Bioactivity

Measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl- KTSAVLQSGFRKME-Edans (Ssynthetic). The specific activity is >13000 pmol/min/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Virus
  • Source E. coli
  • Tag N-6*His
  • Accession

    YP_009725301.1 (S1-Q306)

  • Gene ID
    43740578  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • SARS-CoV-2 3CLpro (S1-Q306)
      Accession # YP_009725301.1
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    M Proteinase; 3CL Proteinase; 2019-nCoV 3C-like Proteinase

  • AA Sequence

    SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ

  • Predicted Molecular Mass

    36.1 kDa

  • Molecular Weight

    34 kDa

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filter solution of PBS, 1 mM EDTA, 10% Glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Sun D, et al. Biochemical characterization of recombinant Avihepatovirus 3C protease and its localization. Virol J. 2019 Apr 29;16(1):54. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • SARS-CoV-2 3CLpro/3C-like protease Protein (His) Product Image
    1/16
  • Experimental Validation Results for SARS-CoV-2 3CLpro/3C-like protease Protein (His)
  • SARS-CoV-2 3CLpro/3C-like protease Protein (His) Product Image