1. Recombinant Proteins
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 3C-like Protease
  5. SARS-CoV-2 3C-like Proteinase (His)

SARS-CoV-2 3C-like Proteinase (His)

Cat. No.: HY-P7429
COA Handling Instructions

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

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  • References

Description

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus[1].

Background

The 3C protease has been regarded as a valid target due to its structural similarity among different picornaviruses and minimal sequence similarity with host proteins; therefore, the development of potent inhibitors against the 3C protease as an antiviral drug is ongoing. Duck hepatitis A virus (DHAV) belongs to the Picornavidea family and is a major threat to the poultry industry[1].

Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl- KTSAVLQSGFRKME-Edans (Ssynthetic). The specific activity is >13000 pmol/min/mg.

Species

Virus

Source

E. coli

Tag

N-6*His

Accession

YP_009725301.1 (S1-Q306)

Gene ID

43740578  [NCBI]

Molecular Construction
N-term
6*His
SARS-CoV-2 3CLpro (S1-Q306)
Accession # YP_009725301.1
C-term
Synonyms
M Proteinase; 3CL Proteinase; 2019-nCoV 3C-like Proteinase
AA Sequence

HHHHHHSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ

Molecular Weight

Approximately 33.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filter solution of PBS, 1 mM EDTA, 10% Glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

SARS-CoV-2 3C-like Proteinase (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SARS-CoV-2 3C-like Proteinase (His)
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HY-P7429
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