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SEPHS1 Protein, Human (HEK293, His)

Cat. No.: HY-P71291
Handling Instructions

The SEPHS1 protein catalyzes the synthesis of selenophosphate, a key step in selenium metabolism involving the conversion of selenide and ATP. This enzyme activity is the basis for selenoprotein biosynthesis and effectively promotes the incorporation of selenium into selenocysteine. SEPHS1 Protein, Human (HEK293, His) is the recombinant human-derived SEPHS1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SEPHS1 Protein, Human (HEK293, His) is 392 a.a., with molecular weight of ~42.0 kDa.

For research use only. We do not sell to patients.

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Description

The SEPHS1 protein catalyzes the synthesis of selenophosphate, a key step in selenium metabolism involving the conversion of selenide and ATP. This enzyme activity is the basis for selenoprotein biosynthesis and effectively promotes the incorporation of selenium into selenocysteine. SEPHS1 Protein, Human (HEK293, His) is the recombinant human-derived SEPHS1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SEPHS1 Protein, Human (HEK293, His) is 392 a.a., with molecular weight of ~42.0 kDa.

Background

The SEPHS1 protein serves as a catalyst in the synthesis of selenophosphate, a crucial step involving the conversion of selenide and ATP. This enzymatic activity demonstrates SEPHS1's pivotal role in selenium metabolism, contributing to the biosynthesis of selenoproteins. By efficiently converting selenide and ATP into selenophosphate, SEPHS1 plays a fundamental part in facilitating the incorporation of selenium into the amino acid selenocysteine, a key component of selenoproteins essential for various cellular functions. The precise enzymatic function of SEPHS1 underscores its significance in selenium homeostasis and the synthesis of biologically active selenoproteins.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P49903 (M1-S392)

Gene ID
Molecular Construction
N-term
SEPHS1 (M1-S392)
Accession # P49903
6*His
C-term
Synonyms
Selenide; water dikinase 1; Selenium donor protein 1; Selenophosphate synthase 1; SEPHS1; SELD; SPS; SPS1
AA Sequence

MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS

Molecular Weight

Approximately 42.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

SEPHS1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SEPHS1 Protein, Human (HEK293, His)
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HY-P71291
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