Serpin A3C Protein, Mouse (HEK293, His)

Serpin A3C Protein, with its reactive center loop (RCL), guides interactions with target proteases. Binding leads to protease cleavage at the RCL, forming a stable serpin-protease complex, inactivating the protease. Variability in Serpin A3C's RCL sequences among Serpina3 paralogs may influence the specificity of target protease interactions, showcasing the protein's adaptability. Serpin A3C Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A3C protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A3C Protein, Mouse (HEK293, His) is 395 a.a., with molecular weight of 50-60 kDa.

Serpin A3C protein features a reactive center loop (RCL) that protrudes from its main body, guiding its interaction with target proteases. Upon binding, the protease cleaves the serpin at the reactive site within the RCL, forming a durable and covalent serpin-protease complex. This complex, marked by the inactivation of the protease, exhibits remarkable stability. The distinct sequences within the RCL of Serpina3 paralogs contribute to the variability, potentially influencing the specificity of target protease interactions.

Measured by its binding ability in a functional ELISA. When Recombinant Mouse SerpinA3C is present at 10 μg/mL, can bind Anti-SerpinA3c Antibody. The ED₅₀ for this effect is 5.374 ng/mL.

Mouse

HEK293

C-His

P29621 (D23-A417)

16625  [NCBI]

DGTLERDTLFHKDKENGTQLDSLTLASINTDFAFSLYKKLALKNPDTNIVFSPLSISAALAIVSLGAKGNTLEEILEGLNFNLTETPEADIHQGFGHLLQRLSHPGEQVQISTGSALFVEKHLQILAEFQEKARALYQAEAFTADFQQPLEATKLINDYVSNQTQRKIKGLISDLDTDTLMVLVNYIYFKGKWKMPFNPRDTFESEFYLDVKRSVKVPMMKIKTLTTPYFRDEELSCTVVELKYKGNASALFILPDQGRMQQVEASLQPETLRKWKNSLRPRKMGELYLPKFSISTDYSLKNILPELGIKEIFSKQADLSGITGTKDLIVSQMVHKAVLDVAETGTEGVAATGVNFRILSRRTSLWFNRTFLMVISHTDVQTTLFIAKITHPKRA

Approximately 55-70 & 115 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.