Copy Link Print Information

Serpin A3C Protein, Mouse (HEK293, His)

Customer Review

Based on 1 Customer Validation

Serpin A3C Protein, with its reactive center loop (RCL), guides interactions with target proteases.Binding leads to protease cleavage at the RCL, forming a stable serpin-protease complex, inactivating the protease.Variability in Serpin A3C's RCL sequences among Serpina3 paralogs may influence the specificity of target protease interactions, showcasing the protein's adaptability.Serpin A3C Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A3C protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Serpin A3C Protein, Mouse (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin A3C Protein, with its reactive center loop (RCL), guides interactions with target proteases.Binding leads to protease cleavage at the RCL, forming a stable serpin-protease complex, inactivating the protease.Variability in Serpin A3C's RCL sequences among Serpina3 paralogs may influence the specificity of target protease interactions, showcasing the protein's adaptability.Serpin A3C Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A3C protein, expressed by HEK293 , with C-His labeled tag.

Background

Serpin A3C protein features a reactive center loop (RCL) that protrudes from its main body, guiding its interaction with target proteases. Upon binding, the protease cleaves the serpin at the reactive site within the RCL, forming a durable and covalent serpin-protease complex. This complex, marked by the inactivation of the protease, exhibits remarkable stability. The distinct sequences within the RCL of Serpina3 paralogs contribute to the variability, potentially influencing the specificity of target protease interactions.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse SerpinA3C is present at 10 μg/mL, can bind Anti-SerpinA3c Antibody. The ED50 for this effect is 5.374 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P29621 (D23-A417)

  • Gene ID
    16625  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin A3C (D23-A417)
      Accession # P29621
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    Serine protease inhibitor A3C; Serpin A3C; Kallikrein-binding protein; KBP; Serpina3c; Klkbp

  • AA Sequence

    DGTLERDTLFHKDKENGTQLDSLTLASINTDFAFSLYKKLALKNPDTNIVFSPLSISAALAIVSLGAKGNTLEEILEGLNFNLTETPEADIHQGFGHLLQRLSHPGEQVQISTGSALFVEKHLQILAEFQEKARALYQAEAFTADFQQPLEATKLINDYVSNQTQRKIKGLISDLDTDTLMVLVNYIYFKGKWKMPFNPRDTFESEFYLDVKRSVKVPMMKIKTLTTPYFRDEELSCTVVELKYKGNASALFILPDQGRMQQVEASLQPETLRKWKNSLRPRKMGELYLPKFSISTDYSLKNILPELGIKEIFSKQADLSGITGTKDLIVSQMVHKAVLDVAETGTEGVAATGVNFRILSRRTSLWFNRTFLMVISHTDVQTTLFIAKITHPKRA

  • Molecular Weight

    Approximately 50-60 kDa & 110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Serpin A3C Protein, Mouse (HEK293, His) Product Image
    1/16
  • Serpin A3C Protein, Mouse (HEK293, His) Product Image