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Serpin B4 Protein, Human (sf9, His)

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Based on 1 publication(s) in Google Scholar

Serpin B4 is a protease inhibitor that may modulate the host immune response against tumor cells, suggesting a crucial role in fine-tuning immune balance. Its ability to inhibit proteases emphasizes its importance in influencing key regulatory pathways, enhancing the immune system's effectiveness against cancer cells. Serpin B4 Protein, Human (sf9, His) is the recombinant human-derived Serpin B4 protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Serpin B4 is a protease inhibitor that may modulate the host immune response against tumor cells, suggesting a crucial role in fine-tuning immune balance. Its ability to inhibit proteases emphasizes its importance in influencing key regulatory pathways, enhancing the immune system's effectiveness against cancer cells. Serpin B4 Protein, Human (sf9, His) is the recombinant human-derived Serpin B4 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

The Serpin B4 protein emerges as a potential regulator in the host immune response against tumor cells by acting as a protease inhibitor. Its role as a modulator suggests a crucial function in fine-tuning the intricate balance of immune responses, particularly in the context of combating tumor cells. The protein's ability to inhibit proteases signifies its capacity to influence key regulatory pathways, potentially enhancing the immune system's effectiveness in recognizing and responding to cancerous cells. Serpin B4, in acting as a protease inhibitor, underscores its significance in the dynamic interplay between the immune system and tumor cells, positioning it as a promising candidate for further exploration in cancer immunotherapy.

Verified Bioactivity

Measured by its ability to inhibit CMA1 cleavage of 100 µM fluorogenic peptide substrate Suc-AAPF-AMC (HY-137811) that incubate at room temperature in kinetic mode for 5 minutes. The IC50 is 15.514 nM.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His
  • Accession

    P48594 (M1-P390)

  • Gene ID
    6318  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • Serpin B4 (M1-P390)
      Accession # P48594
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    SERPINB4; Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 4; Prev. SCCA2; Serpin B4; Squamous Cell Carcinoma Antigen 2; Protease Inhibitor (Leucine-Serpin); SCCA-2; Squamous Cell Carcinoma Antigen 1; PI11; SCCA2/SCCA1 Fusion Protein; LEUPIN; Leupi

  • AA Sequence

    MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFYQTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLVNAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGDADLSGMTWSHGLSVSKVLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNKTNSILFYGRFSSP

  • Predicted Molecular Mass

    47.1 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 20% Glycerol, 1 mM EDTA, 1 mM DTT, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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