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SFRP4 Protein, Human (HEK293, His)

Cat. No.: HY-P74551
COA Handling Instructions

The SFRP4 protein is a member of the soluble Frizzled-related protein (sFRP) and regulates Wnt signaling by directly interacting with Wnt. It has similar functions to other sFRPs and contributes to the regulation of cell growth and differentiation in specific cell types. SFRP4 Protein, Human (HEK293, His) is the recombinant human-derived SFRP4 protein, expressed by HEK293 , with C-His labeled tag. The total length of SFRP4 Protein, Human (HEK293, His) is 329 a.a., with molecular weight of 55-60 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The SFRP4 protein is a member of the soluble Frizzled-related protein (sFRP) and regulates Wnt signaling by directly interacting with Wnt. It has similar functions to other sFRPs and contributes to the regulation of cell growth and differentiation in specific cell types. SFRP4 Protein, Human (HEK293, His) is the recombinant human-derived SFRP4 protein, expressed by HEK293 , with C-His labeled tag. The total length of SFRP4 Protein, Human (HEK293, His) is 329 a.a., with molecular weight of 55-60 kDa.

Background

SFRP4, a member of the soluble frizzled-related proteins (sFRPs), serves as a modulator of Wnt signaling through its direct interaction with Wnts. Demonstrating functional similarities to other sFRPs, SFRP4 contributes to the regulation of cell growth and differentiation in specific cell types. Notably, it plays a crucial role in bone morphogenesis and may act as a regulator of adult uterine morphology and function. Additionally, SFRP4 is implicated in increasing apoptosis during ovulation, potentially through the modulation of FZ1/FZ4/WNT4 signaling. Furthermore, it exhibits phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. These diverse functions underscore the versatile regulatory role of SFRP4 in various cellular processes and physiological contexts.

Biological Activity

Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells and the ED50 is typically 1-6 μg/mL in the presence of 20 ng/mL of Wnt3a.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q6FHJ7 (M18-V346)

Gene ID
Synonyms
Secreted frizzled-related protein 4; sFRP-4; FrpHE; FRPHE
Molecular Weight

55-60 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

SFRP4 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SFRP4 Protein, Human (HEK293, His)
Cat. No.:
HY-P74551
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