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SHP-1 Protein, Human (His)

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Based on 1 publication(s) in Google Scholar

SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Human (His) is the recombinant human-derived SHP-1 protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for SHP-1 Protein, Human (His)
  • SHP-1 Protein, Human (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Human (His) is the recombinant human-derived SHP-1 protein, expressed by E. coli , with C-6*His labeled tag.

Background

SHP-1 protein acts as a critical modulator of signaling pathways initiated by tyrosine phosphorylated cell surface receptors, including KIT and the EGF receptor/EGFR. Additionally, it enhances the inhibition of mast cell activation mediated by the Lilrb4a receptor. The SH2 regions of SHP-1 may engage with other cellular components, influencing its phosphatase activity against interacting substrates. Notably, in collaboration with MTUS1, SHP-1 induces UBE2V2 expression in response to angiotensin II stimulation. Beyond these regulatory functions, SHP-1 plays a key role in hematopoiesis, further emphasizing its significance in the intricate network of cellular processes and signaling events.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    AAH02523.1 (K243-I541)

  • Gene ID
    5777  [NCBI]
  • Molecular Construction
    • N-term
    • SHP-1 (K243-I541)
      Accession # AAH02523.1
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    PTPN6; Protein-Tyrosine Phosphatase SHP-1; Protein Tyrosine Phosphatase Non-Receptor Type 6; Protein-Tyrosine Phosphatase 1C; PTP-1C; SH-PTP1; HCP; Tyrosine-Protein Phosphatase Non-Receptor Type; SHP-1; Hematopoietic Cell Phosphatase; HCPH; Protein-Tyrosi

  • AA Sequence

    KAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNI

  • Predicted Molecular Mass

    35.4 kDa

  • Molecular Weight

    Approximately 34 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl, 500 mM NaCl, 2 mM 2-Mercaptoethanol, 1 mM EDTA, 1 mM DTT, 20% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • SHP-1 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for SHP-1 Protein, Human (His)
  • SHP-1 Protein, Human (His) Product Image