Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc)

Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) is the recombinant human-derived Siglec-11 protein, expressed by CHO, with C-hFc labeled tag.

Siglec-11 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2,8-linked sialic acid, though its sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, Siglec-11 may function as an inhibitory receptor upon ligand-induced tyrosine phosphorylation by recruiting cytoplasmic phosphatases via their SH2 domains, which block signal transduction through dephosphorylation of signaling molecules.

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED₅₀ for this effect is 0.975 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED₅₀ for this effect is 0.975 μg/mL.

Human

CHO

C-hFc

AAK72907 (N17-H543, E84A, K145Q)

114132  [NCBI]

Partial

NKDPSYSLQVQRQVPVPEGLCVIVSCNLSYPRDGWDESTAAYGYWFKGRTSPKTGAPVATNNQSREVAMSTRDRFQLTGDPGKGSCSLVIRDAQREDEAWYFFRVERGSRVRHSFLSNAFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFKKCPAPSFSWTGAALSPRRTRPSTSHFSVLSFTPSPQDHDTDLTCHVDFSRKGVSAQRTVRLRVAYAPKDLIISISHDNTSALELQGNVIYLEVQKGQFLRLLCAADSQPPATLSWVLQDRVLSSSHPWGPRTLGLELRGVRAGDSGRYTCRAENRLGSQQQALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLRLVCVTHSSPPARLSWTRWGQTVGPSQPSDPGVLELPPIQMEHEGEFTCHAQHPLGSQHVSLSLSVHYPPQLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQGSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCKAWNVHGAQSGSVFQLLPGKLEH

Approximately 113-130 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.