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Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc)

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Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) is the recombinant human-derived Siglec-11 protein, expressed by CHO, with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc)
  • Experimental Validation Results for Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc)
  • Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) is the recombinant human-derived Siglec-11 protein, expressed by CHO, with C-hFc labeled tag.

Background

Siglec-11 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2,8-linked sialic acid, though its sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, Siglec-11 may function as an inhibitory receptor upon ligand-induced tyrosine phosphorylation by recruiting cytoplasmic phosphatases via their SH2 domains, which block signal transduction through dephosphorylation of signaling molecules.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED50 for this effect is 0.975 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED50 for this effect is 0.975 μg/mL.

Technical Parameters

  • Species Human
  • Source CHO
  • Tag C-hFc
  • Accession

    AAK72907 (N17-H543, E84A, K145Q)

  • Gene ID
    114132  [NCBI]
  • Molecular Construction
    • N-term
    • Siglec-11 (N17-H543, E84A, K145Q)
      Accession # AAK72907
    • hFc
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    SIGLEC11; Siglec-11; Sialic Acid Binding Ig Like Lectin 11; Sialic Acid Binding Ig-Like Lectin 11; Sialic Acid-Binding Ig-Like Lectin 11; Sialic Acid-Binding Lectin 11

  • AA Sequence

    NKDPSYSLQVQRQVPVPEGLCVIVSCNLSYPRDGWDESTAAYGYWFKGRTSPKTGAPVATNNQSREVAMSTRDRFQLTGDPGKGSCSLVIRDAQREDEAWYFFRVERGSRVRHSFLSNAFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFKKCPAPSFSWTGAALSPRRTRPSTSHFSVLSFTPSPQDHDTDLTCHVDFSRKGVSAQRTVRLRVAYAPKDLIISISHDNTSALELQGNVIYLEVQKGQFLRLLCAADSQPPATLSWVLQDRVLSSSHPWGPRTLGLELRGVRAGDSGRYTCRAENRLGSQQQALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLRLVCVTHSSPPARLSWTRWGQTVGPSQPSDPGVLELPPIQMEHEGEFTCHAQHPLGSQHVSLSLSVHYPPQLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQGSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCKAWNVHGAQSGSVFQLLPGKLEH

  • Molecular Weight

    Approximately 110-135 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED50 for this effect is 0.975 μg/mL.

  • Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) Product Image
    1/16
  • Experimental Validation Results for Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc)
  • Experimental Validation Results for Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc)
  • Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) Product Image