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SLC15A1 Protein, Human (HEK293, Strep, His)

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SLC15A1 Protein, Human (HEK293) is the recombinant human-derived SLC15A1, expressed by HEK293 , with N-Strep, C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • SLC15A1 Protein, Human (HEK293, Strep, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

SLC15A1 Protein, Human (HEK293) is the recombinant human-derived SLC15A1, expressed by HEK293 , with N-Strep, C-His labeled tag.

Background

SLC15A1 is an electrogenic proton-coupled amino-acid transporter that primarily transports oligopeptides of 2 to 4 amino acids, with a preference for dipeptides. It transports neutral and monovalently charged peptides with a proton-to-peptide stoichiometry of 1:1 or 2:1 (By similarity). This protein is mainly responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen (By similarity) and mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides, contributing to the mucosal immune system's recognition of pathogenic bacteria (PubMed:15521010, PubMed:9835627).

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-StrepⅡ;C-His
  • Accession

    P46059 (G2-M708)

  • Gene ID
    6564
  • Protein Length

    Full Length

  • Synonyms

    SLC15A1; Intestinal H(+)/Peptide Cotransporter; Solute Carrier Family 15 Member 1; BA551M18.1.1 (Solute Carrier Family 15 (Oligopeptide Transporter) Member 1); PEPT1; Solute Carrier Family 15 Oligopeptide Transporter Member 1; Peptide Transporter 1; Macro

  • AA Sequence

    GMSKSHSFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDHNHDGTPDSLPVHVVLSLIGLALIALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTIITPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCIGFAIKNRFRHRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQGSRWTLQATTMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCGFNFTSLKKMAVGMVLASMAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGPMSQTNAFMTFDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQVVKDGLNQKPEKGENGIRFVNTFNELITITMSGKVYANISSYNASTYQFFPSGIKGFTISSTEIPPQCQPNFNTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQYFLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFSKQWAEYILFAALLLVVCVIFAIMARFYTYINPAEIEAQFDEDEKKNRLEKSNPYFMSGANSQKQM

  • Predicted Molecular Mass

    82.2 kDa

  • Molecular Weight

    Approximately 72 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM HEPES , pH 7.5, 150 mM NaCl, 0.03% (w/v) GDN, 0.003% (w/v) CHS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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