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  5. ST6GAL1 Protein, Mouse (HEK293, His)

ST6GAL1 Protein, Mouse (HEK293, His)

Cat. No.: HY-P76094
COA Handling Instructions

ST6GAL1 Protein catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing acceptor substrates. ST6GAL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ST6GAL1 protein, expressed by HEK293 , with N-His labeled tag. The total length of ST6GAL1 Protein, Mouse (HEK293, His) is 377 a.a., with molecular weight of 50-55 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

ST6GAL1 Protein catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing acceptor substrates. ST6GAL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ST6GAL1 protein, expressed by HEK293 , with N-His labeled tag. The total length of ST6GAL1 Protein, Mouse (HEK293, His) is 377 a.a., with molecular weight of 50-55 kDa.
Background

ST6GAL1 protein functions as a catalyst in the transfer of sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Biological Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is > 150 pmol/min/μg.
Species

Mouse
Source

HEK293
Tag

N-His
Accession

Q64685 (K27-C403)
Gene ID

20440  [NCBI]
Molecular Construction
N-term
His
ST6GAL1 (K27-C403)
Accession # Q64685
C-term
Synonyms
Beta-Galactoside Alpha-2; 6-Sialyltransferase 1; Sialyltransferase 1; ST6GAL1; SIAT1
Molecular Weight

50-55 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Appearance

Solution.
Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris,120 mM NaCl, pH7.5.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

ST6GAL1 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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