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STC2/Stanniocalcin-2 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

STC2/Stanniocalcin-2 Protein plays a vital role in maintaining calcium and phosphate homeostasis and exhibits strong anti-hypocalcemic effects. As a homodimer with disulfide linkages, STC2 plays a crucial structural role in regulating calcium and phosphate balance. This unique property emphasizes the pivotal role of STC2 in preserving the delicate equilibrium of essential minerals in the body. STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is the recombinant human-derived STC2/Stanniocalcin-2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for STC2/Stanniocalcin-2 Protein, Human (HEK293, His)
  • Experimental Validation Results for STC2/Stanniocalcin-2 Protein, Human (HEK293, His)
  • STC2/Stanniocalcin-2 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

STC2/Stanniocalcin-2 Protein plays a vital role in maintaining calcium and phosphate homeostasis and exhibits strong anti-hypocalcemic effects. As a homodimer with disulfide linkages, STC2 plays a crucial structural role in regulating calcium and phosphate balance. This unique property emphasizes the pivotal role of STC2 in preserving the delicate equilibrium of essential minerals in the body. STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is the recombinant human-derived STC2/Stanniocalcin-2 protein, expressed by HEK293 , with C-His labeled tag.

Background

STC2, a crucial player in calcium and phosphate homeostasis, exerts a potent anti-hypocalcemic action. Operating as a homodimer with disulfide linkages, STC2 showcases its structural significance in mediating regulatory responses related to the balance of calcium and phosphate levels. This distinctive property underscores the pivotal role of STC2 in maintaining the intricate equilibrium of essential minerals within the physiological milieu.

Verified Bioactivity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 23.21 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a, corresponding to a specific activity is 4.308×104 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    O76061 (T25-R302)

  • Gene ID
    8614  [NCBI]
  • Molecular Construction
    • N-term
    • STC2 (T25-R302)
      Accession # O76061
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    STC2; Stanniocalcin-Related Protein; Stanniocalcin 2; STC-Related Protein; STC-2; STCRP; Stanniocalcin-2

  • AA Sequence

    TDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQVQCEQNWGSLCSILSFCTSAIQKPPTAPPERQPQVDRTKLSRAHHGEAGHHLPEPSSRETGRGAKGERGSKSHPNAHARGRVGGLGAQGPSGSSEWEDEQSEYSDIRR

  • Predicted Molecular Mass

    30.7 kDa

  • Molecular Weight

    Approximately 33-43 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 23.21 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a, corresponding to a specific activity is 4.308×104 U/mg

  • STC2/Stanniocalcin-2 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for STC2/Stanniocalcin-2 Protein, Human (HEK293, His)
  • Experimental Validation Results for STC2/Stanniocalcin-2 Protein, Human (HEK293, His)
  • STC2/Stanniocalcin-2 Protein, Human (HEK293, His) Product Image