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TAK1-TAB1 Protein, Human (sf9)

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Based on 1 publication(s) in Google Scholar

TAK1-TAB1 fusion protein is a constitutively active mitogen-activated protein kinase kinase that activates the AP-1 and NF-κB signaling pathways. TAK1-TAB1, Human (sf9) is a recombinant human TAK1-TAB1 protein expressed by sf9 without a tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

TAK1-TAB1 fusion protein is a constitutively active mitogen-activated protein kinase kinase that activates the AP-1 and NF-κB signaling pathways. TAK1-TAB1, Human (sf9) is a recombinant human TAK1-TAB1 protein expressed by sf9 without a tag.

Background

TAK1 regulates c-Jun N-terminal kinase (JNK)/p38 MAPKs and IκB kinase (IKK) signaling pathways, leading to the activation of transcription factors AP-1 and NF-κB. In addition, TAK1 is a negative regulator of the Wnt signaling pathway, in which TAK1 downregulates transcription mediated by a complex of b-catenin and T-cell factor/lymphoid enhancer factor. Two functionally important TAK1-binding proteins, TAB1 and TAB2, are identified to date. TAB1 is a specific activator of TAK1 and associates with the Nterminal kinase domain of TAK1. The TAK1-activation domain consisting of C-terminal 68 amino acids of TAB1 is sufficient for the association with and the activation of TAK1[1].

Verified Bioactivity

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag Tag Free
  • Accession

    O43318-1 (I31-Q303)&Q15750-1 (H468-E497)

  • Gene ID
    6885
  • Protein Length

    Partial

  • Synonyms

    MAP3K7; TGF-Beta Activated Kinase 1; Prev. TAK1; TGF1a; MEKK7; CSCF; Transforming Growth Factor-Beta-Activated Kinase 1; FMD2; Mitogen-Activated Protein Kinase Kinase Kinase 7; TGF-Beta-Activated Kinase 1; TAB1; TGF-Beta-Activated Kinase 1-Binding Protein

  • Predicted Molecular Mass

    34.3 kDa

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 5 mM DTT,0.1M trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Sakurai H, et al. TAK1-TAB1 fusion protein: a novel constitutively active mitogen-activated protein kinase kinase kinase that stimulates AP-1 and NF-kappaB signaling pathways. Biochem Biophys Res Commun. 2002 Oct 11;297(5):1277-81. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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