Tapasin Protein, Human (HEK293, His)
Based on 1 Customer Validation
Tapasin protein is key to MHC class I assembly, binding to TAP and forming MHC class I with peptides. As a heterodimer of PDIA3, tapasin mediates interactions between MHC class I, calreticulin, PDIA3, and TAP. Tapasin Protein, Human (HEK293, His) is the recombinant human-derived Tapasin protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Tapasin protein is key to MHC class I assembly, binding to TAP and forming MHC class I with peptides. As a heterodimer of PDIA3, tapasin mediates interactions between MHC class I, calreticulin, PDIA3, and TAP. Tapasin Protein, Human (HEK293, His) is the recombinant human-derived Tapasin protein, expressed by HEK293 , with C-His labeled tag.
Background
Tapasin protein assumes a pivotal role in the intricate processes of MHC class I assembly, specifically in the association with transporter associated with antigen processing (TAP) and the formation of MHC class I with peptides during peptide loading. Forming a heterodimer with PDIA3 through disulfide linkage, tapasin becomes an obligatory mediator in the interaction among newly assembled MHC class I molecules, calreticulin, PDIA3, and TAP. Notably, up to four MHC class I/tapasin complexes bind to a single TAP, as documented in various studies. Furthermore, tapasin exhibits an additional interaction with the HLA-G-B2M complex, a critical engagement that is essential for the loading of high-affinity peptides, elucidating its multifaceted role in the orchestration of MHC class I antigen presentation.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
O15533 (G21-V414)
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Molecular Construction
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N-term
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Tapasin (G21-V414)
Accession # O15533 -
His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
TAPBP; TAP Binding Protein (Tapasin); TAP Binding Protein; TAP-Associated Protein; Tapasin; TAP-Binding Protein; NGS17; NGS-17; TAPA; TAPBP Protein; TPSN; MHC1D3; TPN
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AA Sequence
GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPTVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSV
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Predicted Molecular Mass
42 kDa
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Molecular Weight
Approximately 45-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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