Tau/MAPT Protein, Mouse (HEK293, His)

Microtubule-associated protein tau (MAPT) plays a pivotal role in promoting microtubule assembly and stability, indicating its potential contribution to neuronal polarity establishment. The C-terminus binds to axonal microtubules, while the N-terminus interacts with neural plasma membrane components, serving as a crucial linker protein between these structures. Tau's localization within the neuronal cell, particularly in the centrosome-defined domain, dictates axonal polarity. Short MAPT isoforms enhance cytoskeletal plasticity, while longer isoforms stabilize it. MAPT engages in diverse interactions with proteins like MARK1, MARK2, MARK3, MARK4, SQSTM1, PSMC2, FKBP4, CSNK1D, SGK1, EPM2A, PIN1, LRRK2, and LRP1, participating in processes from ubiquitination to dephosphorylation and endocytosis, underscoring its multifaceted role in cellular dynamics. Tau/MAPT Protein, Mouse (HEK293, His) is the recombinant mouse-derived Tau/MAPT protein, expressed by HEK293, with N-10*His labeled tag. The total length of Tau/MAPT Protein, Mouse (HEK293, His) is 732 a.a., with molecular weight of 78.9 kDa.

Microtubule-associated protein tau (MAPT) is a key player in the promotion of microtubule assembly and stability, suggesting its potential involvement in the establishment and maintenance of neuronal polarity. Its C-terminus binds to axonal microtubules, while the N-terminus interacts with neural plasma membrane components, indicating tau's role as a crucial linker protein bridging these cellular structures. The predetermined axonal polarity is dictated by tau's localization within the neuronal cell, specifically in the domain defined by the centrosome. Short isoforms of MAPT contribute to the plasticity of the cytoskeleton, whereas longer isoforms may preferentially play a role in its stabilization. MAPT engages in diverse interactions with various proteins such as MARK1, MARK2, MARK3, MARK4, SQSTM1, PSMC2, FKBP4, CSNK1D, SGK1, EPM2A, PIN1, LRRK2, and LRP1, participating in processes ranging from ubiquitination to dephosphorylation and endocytosis. These intricate interactions highlight MAPT's multifaceted involvement in cellular dynamics.

Mouse

HEK293

N-10*His

P10637 (A2-L733)

17762  [NCBI]

ADPRQEFDTMEDHAGDYTLLQDQEGDMDHGLKESPPQPPADDGAEEPGSETSDAKSTPTAEDVTAPLVDERAPDKQAAAQPHTEIPEGITAEEAGIGDTPNQEDQAAGHVTQGRREGQAPDLGTSDWTRQQVSSMSGAPLLPQGLREATCQPSGTRPEDIEKSHPASELLRRGPPQKEGWGQDRLGSEEEVDEDLTVDESSQDSPPSQASLTPGRAAPQAGSGSVCGETASVPGLPTEGSVPLPADFFSKVSAETQASQPEGPGTGPMEEGHEAAPEFTFHVEIKASTPKEQDLEGATVVGVPGEEQKAQTQGPSVGKGTKEASLQEPPGKQPAAGLPGRPVSRVPQLKARVASKDRTGNDEKKAKTSTPSCAKAPSHRPCLSPTRPTLGSSDPLIKPSSPAVSPEPATSPKHVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAASPAQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

78.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.