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TetR Protein, E.coli (His-SUMO)

Cat. No.: HY-P71520
COA Handling Instructions

TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His-SUMO) is the recombinant E. coli-derived TetR protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $280 In-stock
50 μg $615 In-stock
100 μg $985 In-stock
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Description

TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His-SUMO) is the recombinant E. coli-derived TetR protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

Background

The TetR protein operates as a repressor for the tetracycline resistance element, with its N-terminal region adopting a helix-turn-helix structure that facilitates DNA binding. Tetracycline binding to TetR induces a conformational change, diminishing the repressor's affinity for the operator sites of the tetracycline resistance gene (tetA) promoter. This dynamic interaction between TetR and tetracycline serves as a regulatory mechanism, modulating the expression of genes associated with tetracycline resistance. In the presence of tetracycline, TetR undergoes alterations in its binding properties, allowing for the derepression of the tetA promoter and enabling the expression of genes involved in resistance to tetracycline.

Species

E.coli

Source

E. coli

Tag

N-SUMO;N-6*His

Accession

P04483 (M1-S207)

Gene ID

56882117  [NCBI]

Molecular Construction
N-term
6*His-SUMO
TetR (M1-S207)
Accession # P04483
C-term
Synonyms
tetR; Tetracycline repressor protein class B from transposon Tn10
AA Sequence

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS

Molecular Weight

Approximately 42 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TetR Protein, E.coli (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TetR Protein, E.coli (His-SUMO)
Cat. No.:
HY-P71520
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