TIMP-1 Protein, Human (HEK293)

TIMP-1 protein refers to tissue inhibitor of metalloproteinases-1, belonging to matrix metalloproteinases (MMPs) regulators. TIMP-1 interacts with CD74, a receptor for macrophage migration inhibitory factor (MIF). And TIMP-1 activates the phosphorylation of AKT and ERK1/2, mediates serine/threonine kinases activation. Thus, TIMP-1-CD74 axis promotes the recruitment response of primary human monocytes. TIMP-1 can be used in research in vascular inflammation and atherosclerosis^[1].

TIMP-1 (12.5-400 ng/mL; 20 min) increases the the phosphorylation level of AKT and ERK1/2 in THP-1 monocytic cells. Moreover, TIMP-1 (100 ng/mL) also promotes the activation of serine/threonine kinases in THP-1 cells^[1].
TIMP-1 also can stimulates THP cells to mediate cell migration and CD74 internalization (50-200 ng/mL; 1 h), with an optimal concentration of 100 ng/mL^[1].

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2 and the IC₅₀ value is < 6 nM.

Human

HEK293

Tag Free

P01033 (C24-A207)

7076  [NCBI]

Approximately 26 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAC, 200 mM NaCl, pH 5.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ebert S, et al. Tissue Inhibitor of Metalloproteinases-1 Interacts with CD74 to Promote AKT Signaling, Monocyte Recruitment Responses, and Vascular Smooth Muscle Cell Proliferation. Cells. 2023 Jul 20;12(14):1899.  [Content Brief]