TKT Protein, Human (His)
Based on 1 Customer Validation
TKT proteins are essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. TKT Protein, Human (His) is the recombinant human-derived TKT protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TKT proteins are essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. TKT Protein, Human (His) is the recombinant human-derived TKT protein, expressed by E. coli , with N-6*His labeled tag.
Background
TKT protein is essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. The enzymatic activity of TKT protein plays a critical role in various metabolic pathways, including the pentose phosphate pathway and the Calvin cycle. The transfer of the ketol group is a key step in these pathways, enabling the conversion of sugars and the generation of important intermediates for energy production and biosynthesis. TKT protein's function is crucial for maintaining metabolic flux and contributing to the overall balance of carbohydrate metabolism in the cell.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P29401 (E2-A623)
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Gene ID7086
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Molecular Construction
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N-term
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6*His
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TKT (E2-A623)
Accession # P29401 -
C-term
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Protein Length
Full Length
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Synonyms
TKT; Transketolase Isoform 3; Transketolase; Transketolase Isoform 2; TK; HEL-S-48; Epididymis Secretory Protein Li 48; HEL107; Epididymis Luminal Protein 107; SDDHD; Wernicke-Korsakoff Syndrome; TKT1
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AA Sequence
ESYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKSQDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLAEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCLLGDGELSEGSVWEAMAFASIYKLDNLVAILDINRLGQSDPAPLQHQMDIYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGVEDKESWHGKPLPKNMAEQIIQEIYSQIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA
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Predicted Molecular Mass
69.8 kDa
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Molecular Weight
Approximately 70-75 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
2.Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 500 mM NaCl, 20% glycerol, 1 mM DTT.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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