TLR2 Protein, Mouse (sf9, His, solution)

The TLR2 protein cooperates with LY96 to initiate an immune response against bacterial lipoproteins and cell wall components.It cooperates with TLR1 or TLR6 to activate NF-kappa-B, induce cytokine secretion, and form activation clusters such as TLR2:TLR6:CD14:CD36.TLR2 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived TLR2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag.

TLR2 Protein collaborates with LY96 to initiate the innate immune response against bacterial lipoproteins and microbial cell wall components. Additionally, it cooperates with TLR1 or TLR6 for the response to specific lipopeptides, acting through MYD88 and TRAF6 to activate NF-kappa-B, induce cytokine secretion, and trigger inflammation. TLR2 forms activation clusters, like TLR2:TLR6:CD14:CD36 and TLR2:TLR1:CD14, in response to different ligands, leading to cell surface signaling and subsequent Golgi targeting via a lipid-raft dependent pathway. Notably, TLR2 recognizes M.tuberculosis antigens, including EsxA (ESAT-6), and serves as a major receptor for various M.tuberculosis lipoproteins, influencing antigen-presenting cell functions. Its interactions with TICAM2, LY96, TLR1, TLR6, MYD88, TICAM1, CNPY3, ATG16L1, non-modified M.tuberculosis protein MPT83, PPP1R11, and TIRAP contribute to its diverse roles in the immune response.

Mouse

Sf9 insect cells

C-10*His

Q9QUN7 (M1-Q587)

24088

Partial

MLRALWLFWILVAITVLFSKRCSAQESLSCDASGVCDGRSRSFTSIPSGLTAAMKSLDLSFNKITYIGHGDLRACANLQVLILKSSRINTIEGDAFYSLGSLEHLDLSDNHLSSLSSSWFGPLSSLKYLNLMGNPYQTLGVTSLFPNLTNLQTLRIGNVETFSEIRRIDFAGLTSLNELEIKALSLRNYQSQSLKSIRDIHHLTLHLSESAFLLEIFADILSSVRYLELRDTNLARFQFSPLPVDEVSSPMKKLAFRGSVLTDESFNELLKLLRYILELSEVEFDDCTLNGLGDFNPSESDVVSELGKVETVTIRRLHIPQFYLFYDLSTVYSLLEKVKRITVENSKVFLVPCSFSQHLKSLEFLDLSENLMVEEYLKNSACKGAWPSLQTLVLSQNHLRSMQKTGEILLTLKNLTSLDISRNTFHPMPDSCQWPEKMRFLNLSSTGIRVVKTCIPQTLEVLDVSNNNLDSFSLFLPRLQELYISRNKLKTLPDASLFPVLLVMKIRENAVSTFSKDQLGSFPKLETLEAGDNHFVCSCELLSFTMETPALAQILVDWPDSYLCDSPPRLHGHRLQDARPSVLECHQ

Approximately 65 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5, 1 mM TCEP.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.