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TLR2 Protein, Mouse (sf9, His, solution)

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The TLR2 protein cooperates with LY96 to initiate an immune response against bacterial lipoproteins and cell wall components.It cooperates with TLR1 or TLR6 to activate NF-kappa-B, induce cytokine secretion, and form activation clusters such as TLR2:TLR6:CD14:CD36.TLR2 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived TLR2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

The TLR2 protein cooperates with LY96 to initiate an immune response against bacterial lipoproteins and cell wall components.It cooperates with TLR1 or TLR6 to activate NF-kappa-B, induce cytokine secretion, and form activation clusters such as TLR2:TLR6:CD14:CD36.TLR2 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived TLR2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag.

Background

TLR2 Protein collaborates with LY96 to initiate the innate immune response against bacterial lipoproteins and microbial cell wall components. Additionally, it cooperates with TLR1 or TLR6 for the response to specific lipopeptides, acting through MYD88 and TRAF6 to activate NF-kappa-B, induce cytokine secretion, and trigger inflammation. TLR2 forms activation clusters, like TLR2:TLR6:CD14:CD36 and TLR2:TLR1:CD14, in response to different ligands, leading to cell surface signaling and subsequent Golgi targeting via a lipid-raft dependent pathway. Notably, TLR2 recognizes M.tuberculosis antigens, including EsxA (ESAT-6), and serves as a major receptor for various M.tuberculosis lipoproteins, influencing antigen-presenting cell functions. Its interactions with TICAM2, LY96, TLR1, TLR6, MYD88, TICAM1, CNPY3, ATG16L1, non-modified M.tuberculosis protein MPT83, PPP1R11, and TIRAP contribute to its diverse roles in the immune response.

Technical Parameters

  • Species Mouse
  • Source Sf9 insect cells
  • Tag C-10*His
  • Accession

    Q9QUN7 (M1-Q587)

  • Gene ID
    24088
  • Molecular Construction
    • N-term
    • TLR2 (M1-Q587)
      Accession # Q9QUN7
    • 10*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    TLR2; CD282; Toll Like Receptor 2; Toll/Interleukin-1 Receptor-Like Protein 4; Toll-Like Receptor 2; Toll-Like Receptor; TIL4; CD282 Antigen

  • AA Sequence

    MLRALWLFWILVAITVLFSKRCSAQESLSCDASGVCDGRSRSFTSIPSGLTAAMKSLDLSFNKITYIGHGDLRACANLQVLILKSSRINTIEGDAFYSLGSLEHLDLSDNHLSSLSSSWFGPLSSLKYLNLMGNPYQTLGVTSLFPNLTNLQTLRIGNVETFSEIRRIDFAGLTSLNELEIKALSLRNYQSQSLKSIRDIHHLTLHLSESAFLLEIFADILSSVRYLELRDTNLARFQFSPLPVDEVSSPMKKLAFRGSVLTDESFNELLKLLRYILELSEVEFDDCTLNGLGDFNPSESDVVSELGKVETVTIRRLHIPQFYLFYDLSTVYSLLEKVKRITVENSKVFLVPCSFSQHLKSLEFLDLSENLMVEEYLKNSACKGAWPSLQTLVLSQNHLRSMQKTGEILLTLKNLTSLDISRNTFHPMPDSCQWPEKMRFLNLSSTGIRVVKTCIPQTLEVLDVSNNNLDSFSLFLPRLQELYISRNKLKTLPDASLFPVLLVMKIRENAVSTFSKDQLGSFPKLETLEAGDNHFVCSCELLSFTMETPALAQILVDWPDSYLCDSPPRLHGHRLQDARPSVLECHQ

  • Predicted Molecular Mass

    64.8 kDa

  • Molecular Weight

    Approximately 65 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5, 1 mM TCEP.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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