TLR3 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
TLR3 protein is an important component of innate and adaptive immunity and can recognize pathogen-associated molecular patterns and activate NF-κ-B, IRF3 and cytokine secretion. Ligand binding induces TLR3 dimerization, and efficient signal transduction requires clustering of signaling units along the ligand. TLR3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TLR3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TLR3 protein is an important component of innate and adaptive immunity and can recognize pathogen-associated molecular patterns and activate NF-κ-B, IRF3 and cytokine secretion. Ligand binding induces TLR3 dimerization, and efficient signal transduction requires clustering of signaling units along the ligand. TLR3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TLR3 protein, expressed by HEK293 , with C-His labeled tag.
Background
TLR3 protein serves as a key component in both innate and adaptive immunity, playing a pivotal role in the host immune response against pathogens by recognizing specific molecular patterns associated with microorganisms. Activated by double-stranded RNA, a characteristic feature of viral infection, TLR3 functions through the adapter TRIF/TICAM1, leading to NF-kappa-B activation, nuclear translocation of IRF3, cytokine secretion, and initiation of the inflammatory response. TLR3 exists as a monomer and homodimer, with ligand-binding triggering dimerization. The signaling unit comprises one approximately 40 bp ds-RNA and two TLR3 molecules, requiring lateral clustering of signaling units along the ds-RNA ligand for effective signal transduction. Interaction with UNC93B1, SRC, and TICAM1, particularly in response to poly(I:C), contributes to its regulatory functions, highlighting the intricate molecular mechanisms involved in TLR3-mediated immune responses.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
Q99MB1/NP_569054.2 (T26-L705)
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Molecular Construction
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N-term
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TLR3 (T26-L705)
Accession # Q99MB1/NP_569054.2 -
His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
TLR3; CD283 Antigen; Toll Like Receptor 3; TLR3 Protein; Toll-Like Receptor 3; IIAE2; CD283; IMD83; Toll-Like Receptor 3, Isoform CRA_b
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AA Sequence
TNQCTVRYNVADCSHLKLTHIPDDLPSNITVLNLTHNQLRRLPPTNFTRYSQLAILDAGFNSISKLEPELCQILPLLKVLNLQHNELSQISDQTFVFCTNLTELDLMSNSIHKIKSNPFKNQKNLIKLDLSHNGLSSTKLGTGVQLENLQELLLAKNKILALRSEELEFLGNSSLRKLDLSSNPLKEFSPGCFQTIGKLFALLLNNAQLNPHLTEKLCWELSNTSIQNLSLANNQLLATSESTFSGLKWTNLTQLDLSYNNLHDVGNGSFSYLPSLRYLSLEYNNIQRLSPRSFYGLSNLRYLSLKRAFTKQSVSLASHPNIDDFSFQWLKYLEYLNMDDNNIPSTKSNTFTGLVSLKYLSLSKTFTSLQTLTNETFVSLAHSPLLTLNLTKNHISKIANGTFSWLGQLRILDLGLNEIEQKLSGQEWRGLRNIFEIYLSYNKYLQLSTSSFALVPSLQRLMLRRVALKNVDISPSPFRPLRNLTILDLSNNNIANINEDLLEGLENLEILDFQHNNLARLWKRANPGGPVNFLKGLSHLHILNLESNGLDEIPVGVFKNLFELKSINLGLNNLNKLEPFIFDDQTSLRSLNLQKNLITSVEKDVFGPPFQNLNSLDMRFNPFDCTCESISWFVNWINQTHTNISELSTHYLCNTPHHYYGFPLKLFDTSSCKDSAPFEL
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Predicted Molecular Mass
78.6 kDa
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Molecular Weight
Approximately 85-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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