TOLLIP Protein, Human (His)

TOLLIP plays a key role in IL-1 and Toll-like receptor signaling, recruiting IRAK1 to the IL-1 receptor complex and inhibiting IRAK1 phosphorylation. It connects the ubiquitin pathway to autophagy, serving as a ubiquitin-ATG8 linker for autophagic clearance of ubiquitin conjugates. TOLLIP Protein, Human (His) is the recombinant human-derived TOLLIP protein, expressed by E. coli , with C-6*His labeled tag. The total length of TOLLIP Protein, Human (His) is 273 a.a., with molecular weight of ~31.0 kDa.

TOLLIP, a key component of the signaling pathway for IL-1 and Toll-like receptors, exerts regulatory functions in cell activation by microbial products. It acts by recruiting IRAK1 to the IL-1 receptor complex, subsequently inhibiting IRAK1 phosphorylation and kinase activity. Additionally, TOLLIP plays a crucial role in connecting the ubiquitin pathway to autophagy, functioning as a ubiquitin-ATG8 family adapter and facilitating the autophagic clearance of ubiquitin conjugates. The selective autophagy pathway, dependent on TOLLIP, is particularly noteworthy for its involvement in the clearance of cytotoxic polyQ proteins aggregates. Furthermore, TOLLIP forms a complex with TOM1, facilitating the recruitment of ubiquitin-conjugated proteins onto early endosomes. It interacts with various proteins, including TLR2, the TLR4-MD2 complex, and ATG8 family proteins, highlighting its diverse roles in cellular processes. Additionally, TOLLIP interacts with phosphatidylinositol 3-phosphate (PtdIns(3)P) and oligomerizes, contributing to its multifaceted functionality in cellular homeostasis.

Human

E. coli

C-6*His

Q9H0E2 (A2-P274)

54472  [NCBI]

ATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP

Approximately 31.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.