TRA2B Protein, Human (sf9, His-Myc)
The TRA2B protein is a sequence-specific RNA-binding factor that complexly regulates pre-mRNA splicing and modulates exon inclusion. It cooperates with RBMX to promote the inclusion of exon 7 in SMN2, demonstrating diverse regulatory functions. TRA2B Protein, Human (sf9, His-Myc) is the recombinant human-derived TRA2B protein, expressed by sf9 insect cells , with N-10*His, C-Myc labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The TRA2B protein is a sequence-specific RNA-binding factor that complexly regulates pre-mRNA splicing and modulates exon inclusion. It cooperates with RBMX to promote the inclusion of exon 7 in SMN2, demonstrating diverse regulatory functions. TRA2B Protein, Human (sf9, His-Myc) is the recombinant human-derived TRA2B protein, expressed by sf9 insect cells , with N-10*His, C-Myc labeled tag.
Background
TRA2B protein, a sequence-specific RNA-binding factor, intricately modulates pre-mRNA splicing, exhibiting the capability to either activate or suppress exon inclusion. In collaboration with RBMX, it plays a pivotal role in promoting exon 7 inclusion of the survival motor neuron SMN2, showcasing its diverse regulatory functions. Additionally, TRA2B takes part in the splicing of MAPT/Tau exon 10, showcasing its involvement in shaping pre-mRNA splicing patterns. By counteracting the effects of splicing regulators like RBMX and binding to the AG-rich SE2 domain in SMN exon 7 RNA, TRA2B exerts a nuanced influence on RNA processing. It is an integral part of a pre-mRNA exonic splicing enhancer (ESE) complex, alongside TRA2B/SFRS10, SNRNP70, SNRPA1, and SRRM1. Further interactions with A3 enhancer proteins SFRS4, SFRS5, SFRS6, and SFRS9, as well as binding to CPSF6, RBMY1A1, RBMX, RNPS1, and phosphorylated SFRS13A, underscore the intricate network through which TRA2B modulates splicing. Its interactions with SAFB/SAFB1, ILDR1, and ILDR2 further contribute to the multifaceted regulatory landscape of TRA2B in RNA processing.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-10*His;C-Myc
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Accession
P62995 (S2-Y288)
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Molecular Construction
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N-term
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10*His
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TRA2B (S2-Y288)
Accession # P62995 -
Myc
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C-term
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Protein Length
Full Length
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Synonyms
TRA2B; TRA-2 Beta; Prev. SFRS10; Transformer 2 Beta Homolog (Drosophila); Htra2-Beta; HTRA2-Beta; PPP1R156; TRA2-BETA; Splicing Factor, Arginine/Serine-Rich 10 (Transformer 2 Homolog, Drosophila); TRA2-Beta; Protein Phosphatase 1, Regulatory Subunit 156;
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AA Sequence
SDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRSRSYSPRRY
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Predicted Molecular Mass
37.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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