TRADD Protein, Human (GST)

TRADD protein acts as an adapter for TNFRSF1A/TNFR1, mediating its interaction with FADD. TRADD induces TNF-induced responses: apoptosis and NF-kappa-B activation. The nuclear form of TRADD functions as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A. Stimulation of TNFRSF1A forms plasma membrane-bound complex I, promoting cell survival. Subsequently, TRADD dissociates to form cytoplasmic complex II, inducing apoptosis. TRADD interacts with various proteins in these complexes, emphasizing its multifaceted role in cellular processes. TRADD Protein, Human (GST) is the recombinant human-derived TRADD protein, expressed by E. coli, with N-GST labeled tag. The total length of TRADD Protein, Human (GST) is 312 a.a., with molecular weight of 61.2 kDa.

The TRADD protein serves as an adapter molecule for TNFRSF1A/TNFR1, specifically associating with the cytoplasmic domain of activated TNFRSF1A/TNFR1 to mediate its interaction with FADD. Overexpression of TRADD induces two major TNF-induced responses: apoptosis and activation of NF-kappa-B. The nuclear form of TRADD acts as a tumor suppressor by preventing the ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A through interaction with TRIP12, disrupting the interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. Stimulation of TNF-alpha receptor TNFRSF1A leads to the formation of two distinct signaling complexes. Plasma membrane-bound complex I, composed of TNFRSF1A, TRADD, RIPK1, TRAF2, and BIRC2/c-IAP1 or BIRC3, interacts with CHUCK/IKK-alpha, IKBKB/IKK-beta, and IKBKG/IKK-gamma, promoting cell survival. Subsequently, TRADD, RIPK1, and TRAF2 dissociate from TNFRSF1A to form cytoplasmic complex II with FADD and caspase CASP8, promoting cell apoptosis. TRADD also interacts with various proteins within these complexes, including TRPC4AP, UXT1, DAB2IP, SQSTM1, TRIP12, HIPK2, KRT14, KRT18, KRT16, KRT17, FADD, and TOMM70, highlighting its multifaceted role in diverse cellular processes.

Human

E. coli

N-GST

Q15628 (M1-A312)

8717

61.2kDa

Greater than 90 % as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.