TRIM16 Protein, Human (sf9, His, StrepⅡ)
TRIM16 protein is an essential E3 ubiquitin ligase that participates in autophagy reaction and ubiquitination during lysosome and phagosome damage. TRIM16 Protein, Human (sf9, His, Strep) is the recombinant human-derived TRIM16 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TRIM16 protein is an essential E3 ubiquitin ligase that participates in autophagy reaction and ubiquitination during lysosome and phagosome damage. TRIM16 Protein, Human (sf9, His, Strep) is the recombinant human-derived TRIM16 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
Background
TRIM16, functioning as an E3 ubiquitin ligase, assumes a crucial role in orchestrating the autophagic response and ubiquitination processes in the face of lysosomal and phagosomal damages. Notably, it contributes to the stress-induced biogenesis and degradation of protein aggresomes by intricately regulating the p62-KEAP1-NRF2 signaling axis. A central aspect of its functionality lies in modulating ubiquitination levels and, consequently, the stability of NRF2, a key player in cellular stress responses. As a scaffold protein, TRIM16 facilitates the autophagic degradation of protein aggregates, forming interactions with p62/SQSTM, ATG16L1, and LC3B/MAP1LC3B. This multifaceted role positions TRIM16 as a guardian against oxidative stress-induced cell death, particularly in response to endomembrane damage.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-StrepⅡ;N-8*His
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Accession
O95361 (A2-P564)
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Gene ID/
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Molecular Construction
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N-term
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8*His-StrepⅡ
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TRIM16 (A2-P564)
Accession # O95361 -
C-term
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Protein Length
Full Length
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Synonyms
TRIM16; EBBP; Tripartite motif-containing protein 16; EC 2.3.2.27; E3 ubiquitin-protein ligase TRIM16; Estrogen-responsive B box protein; Tripartite Motif-Containing 16; CDNA FLJ31464 Fis, Clone NT2NE2001337, Highly Similar To Tripartite Motif-Containing
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AA Sequence
AELDLMAPGPLPRATAQPPAPLSPDSGSPSPDSGSASPVEEEDVGSSEKLGRETEEQDSDSAEQGDPAGEGKEVLCDFCLDDTRRVKAVKSCLTCMVNYCEEHLQPHQVNIKLQSHLLTEPVKDHNWRYCPAHHSPLSAFCCPDQQCICQDCCQEHSGHTIVSLDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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