1. Recombinant Proteins
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Trk Receptors TrkA
  5. TrkA Protein, Rat (HEK293, His)

TrkA Protein, Rat (HEK293, His)

Cat. No.: HY-P73460
SDS COA Handling Instructions

The TrkA protein is a receptor tyrosine kinase that is critical in central and peripheral nervous system development, regulating neuronal proliferation, differentiation, and survival. As a high-affinity NGF receptor, TrkA undergoes homodimerization and autophosphorylation upon ligand binding, activating downstream effectors (SHC1, FRS2, SH2B1, SH2B2, PLCG1) cascade. TrkA Protein, Rat (HEK293, His) is the recombinant rat-derived TrkA protein, expressed by HEK293 , with C-His labeled tag. The total length of TrkA Protein, Rat (HEK293, His) is 386 a.a., with molecular weight of ~70-95 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The TrkA protein is a receptor tyrosine kinase that is critical in central and peripheral nervous system development, regulating neuronal proliferation, differentiation, and survival. As a high-affinity NGF receptor, TrkA undergoes homodimerization and autophosphorylation upon ligand binding, activating downstream effectors (SHC1, FRS2, SH2B1, SH2B2, PLCG1) cascade. TrkA Protein, Rat (HEK293, His) is the recombinant rat-derived TrkA protein, expressed by HEK293 , with C-His labeled tag. The total length of TrkA Protein, Rat (HEK293, His) is 386 a.a., with molecular weight of ~70-95 kDa.

Background

The TrkA protein, a receptor tyrosine kinase, plays a vital role in the development and maturation of the central and peripheral nervous systems by regulating the proliferation, differentiation, and survival of sympathetic and sensory neurons. Serving as a high-affinity receptor for NGF, its primary ligand, TrkA can also be activated by NTF3/neurotrophin-3, though NTF3 specifically supports axonal extension through NTRK1 without influencing neuron survival. Upon dimeric NGF ligand-binding, TrkA undergoes homodimerization, autophosphorylation, and activation, subsequently recruiting, phosphorylating, and/or activating downstream effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1. These effectors regulate distinct yet overlapping signaling cascades, steering cell survival and differentiation. Through SHC1 and FRS2, TrkA activates a GRB2-Ras-MAPK cascade controlling cell differentiation and survival, while through PLCG1, it modulates NF-Kappa-B activation and the transcription of genes crucial for cell survival. Additionally, through SHC1 and SH2B1, TrkA controls a Ras-PI3 kinase-AKT1 signaling cascade, further contributing to the regulation of cell survival. In the absence of ligand and activation, TrkA may promote cell death, underscoring the dependence of neuron survival on trophic factors.

Biological Activity

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 13.22 ng/mL in the presence of 10 ng/mL of rrNGF, corresponding to a specific activity is 7.564×10^4 U/mg.

  • Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 13.22 ng/mL in the presence of 10 ng/mL of rrNGF,corresponding to a specific activity is 7.564×104 U/mg
Species

Rat

Source

HEK293

Tag

C-His

Accession

P35739 (A33-P418)

Gene ID
Molecular Construction
N-term
TrkA (A33-P418)
Accession # P35739
His
C-term
Synonyms
High affinity nerve growth factor receptor; Trk-A; NTRK1; MTC; TRK
AA Sequence

AASCRETCCPVGPSGLRCTRAGTLNTLRGLRGAGNLTELYVENQRDLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFHFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNNSCGVPSVKIQMPNDSVEVGDDVFLQCQVEGQALQQADWILTELEGTATMKKSGDLPSLGLTLVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGKAVEQHHWCIPFSVDGQPAPSLRWFFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSRDPVEKKDETP

Molecular Weight

Approximately 70-95 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TrkA Protein, Rat (HEK293, His)
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HY-P73460
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