TrkB Protein, Human (HEK293, His)

The TrkB protein is a key receptor tyrosine kinase that plays a key role in central and peripheral nervous system development. It regulates neuronal survival, proliferation, migration, differentiation, synapse formation, and plasticity. TrkB Protein, Human (HEK293, His) is the recombinant human-derived TrkB protein, expressed by HEK293 , with C-6*His labeled tag.

TrkB protein, a receptor tyrosine kinase, plays a critical role in the development and maturation of the central and peripheral nervous systems. It regulates various processes including neuron survival, proliferation, migration, differentiation, synapse formation, and plasticity. TrkB acts as a receptor for BDNF, NTF4, and also has the ability to bind NTF3, although less efficiently. Upon ligand binding, TrkB undergoes homodimerization, autophosphorylation, and activation. This activates downstream effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1, which control distinct signaling cascades. The GRB2-Ras-MAPK cascade, regulated by SHC1, FRS2, SH2B1, and SH2B2, is involved in neuronal differentiation and neurite outgrowth. The Ras-PI3 kinase-AKT1 cascade, also controlled by these effectors, primarily regulates cell growth and survival. TrkB also plays a role in synaptic plasticity and is involved in learning and memory. Through PLCG1, TrkB activates NF-Kappa-B and promotes the transcription of genes associated with cell survival. Furthermore, TrkB is implicated in neutrophin-dependent calcium signaling in glial cells and facilitates communication between neurons and glia. Overall, TrkB is a crucial player in cell survival and various neural processes.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Human BDNF at 1 μg/mL (100 μL/well) can bind Human TrkB Protein. The ED₅₀ for this effect is 17.97 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human BDNF at 1 μg/mL (100 μL/well) can bind Human TrkB Protein. The ED₅₀ for this effect is 17.97 ng/mL.

Human

HEK293

C-6*His

Q16620-1 (C32-H430)

4915  [NCBI]

Extracellular Domain

CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREH

Approximately 60-105 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.