UBE2R1/CDC34 Protein, Human (sf9, His, StrepII)

The UBE2R1/CDC34 protein is an important ubiquitin-proteasome system component that functions as an E2 ubiquitin-conjugating enzyme, capable of proficiently accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to a variety of protein substrates. . In vitro, UBE2R1 efficiently mediates “Lys-48”-linked polyubiquitination. UBE2R1/CDC34 Protein, Human (sf9, His, Strep) is the recombinant human-derived UBE2R1/CDC34 protein, expressed by sf9 insect cells , with N-Strep, N-His labeled tag.

UBE2R1, a pivotal component in the ubiquitin-proteasome system, functions as an E2 ubiquitin-conjugating enzyme, adept at accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to various protein substrates. In vitro, UBE2R1 demonstrates its catalytic prowess by efficiently mediating 'Lys-48'-linked polyubiquitination reactions. Collaborating with specific E3 ligase complexes, such as SCF(FBXW11) and SCF(SKP2), UBE2R1 plays a crucial role in orchestrating the ubiquitin-dependent degradation of key regulatory proteins. Notably, it participates in the polyubiquitination of NFKBIA, leading to its subsequent proteasomal degradation, thereby influencing processes related to cellular proliferation. UBE2R1's involvement extends to the regulation of cell cycle progression, targeting proteins like WEE1 kinase and beta-catenin for ubiquitination and degradation. Moreover, it becomes a target itself for the human herpes virus 1 protein ICP0, establishing dynamic interactions with proteasomes and contributing to the intricate landscape of cellular protein homeostasis.

Incubation of UB2R1 for 60 minutes at 37˚C in the presence of Bodipy-ubiquitin, UBE1 and ATP was compared alongside two control reactions with either ATP or UB2R1excluded from the reaction. Ubiquitin conjugates were identified by the migration of the Bodipy-ubiquitin band and these were observed only in the presence of ATP and UB2R1.

Human

Sf9 insect cells

N-StrepⅡ;N-His

P49427 (A2-S236)

997  [NCBI]

Partial

ARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES

Approximately 31 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 1 mM DTT, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.