USP21 Protein, Human
Based on 1 Customer Validation
USP21 protein is a multifunctional deubiquitinase that plays a key role in the regulation of epigenetic transcriptional repression by deubiquitinating histone H2A. As a coactivator, it relieves the inhibition of dimethylation and trimethylation of histone H3 at "Lys-4", thereby regulating transcription initiation. USP21 Protein, Human is the recombinant human-derived USP21 protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
USP21 protein is a multifunctional deubiquitinase that plays a key role in the regulation of epigenetic transcriptional repression by deubiquitinating histone H2A. As a coactivator, it relieves the inhibition of dimethylation and trimethylation of histone H3 at "Lys-4", thereby regulating transcription initiation. USP21 Protein, Human is the recombinant human-derived USP21 protein, expressed by E. coli , with tag free.
USP21, a multifunctional deubiquitinase, plays a pivotal role in the epigenetic regulation of transcriptional repression by specifically deubiquitinating histone H2A, acting as a coactivator and relieving the repression of di- and trimethylation of histone H3 at 'Lys-4', thus modulating transcriptional initiation. This regulatory function extends to gene expression modulation through histone H2A deubiquitination. Additionally, USP21 stabilizes BAZ2A/TIP5 through deubiquitination, demonstrating its involvement in the maintenance of protein stability. Furthermore, USP21 exhibits the capability to remove NEDD8 from NEDD8 conjugates without affecting Sentrin-1 conjugates. Notably, it acts as a negative regulator of the ribosome quality control (RQC) by mediating the deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby counteracting ZNF598-mediated 40S ubiquitination and influencing the intricate dynamics of cellular processes.
The fundamental role of USP21 is specific removal of ubiquitin from substrates. USP21 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q9UK80 (P2-L565)
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Gene ID27005
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Molecular Construction
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N-term
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USP21 (P2-L565)
Accession # Q9UK80 -
C-term
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Protein Length
Partial
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Synonyms
USP21; Ubiquitin carboxyl-terminal hydrolase 21; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21
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AA Sequence
PQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPASGPNPMLRPLPPRPGLPDERLKKLELGRGRTSGPRPRGPLRADHGVPLPGSPPPTVALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALSRLALRPEPPTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDDKMAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL
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Predicted Molecular Mass
62.9 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
1.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol.
2.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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