USP25 Protein, Human (His)
The USP25 protein acts as a deubiquitinating enzyme and plays a key role in the hydrolysis of the ubiquitin moiety conjugated to the substrate. This function enables processing of newly synthesized ubiquitin, recycling of ubiquitin molecules, and editing of polyubiquitin chains, thus preventing proteasomal degradation of substrates. USP25 Protein, Human (His) is the recombinant human-derived USP25 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The USP25 protein acts as a deubiquitinating enzyme and plays a key role in the hydrolysis of the ubiquitin moiety conjugated to the substrate. This function enables processing of newly synthesized ubiquitin, recycling of ubiquitin molecules, and editing of polyubiquitin chains, thus preventing proteasomal degradation of substrates. USP25 Protein, Human (His) is the recombinant human-derived USP25 protein, expressed by E. coli , with N-6*His labeled tag.
Background
Ubiquitin-specific protease 25 (USP25) is a deubiquitinating enzyme with the capacity to hydrolyze ubiquitin moieties conjugated to substrates, thereby participating in the processing of newly synthesized ubiquitin, recycling ubiquitin molecules, and editing polyubiquitin chains. USP25 plays a crucial role in preventing the proteasomal degradation of substrates by cleaving ubiquitin chains. Notably, it exhibits hydrolytic activity towards both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains, showcasing its versatility in targeting different ubiquitin linkage types. Additionally, a muscle-specific isoform of USP25, known as USP25m, is suggested to play a role in the regulation of muscular differentiation and function, indicating its potential significance in specific cellular contexts.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His
-
Accession
Q9UHP3 (T2-R1055)
-
Gene ID29761
-
Molecular Construction
-
N-term
-
6*His
-
USP25 (T2-R1055)
Accession # Q9UHP3 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
USP25; Ubiquitin Specific Peptidase 25; USP21; Ubiquitin Carboxyl-Terminal Hydrolase 25; Deubiquitinating Enzyme 25; Ubiquitin Thioesterase 25; USP On Chromosome 21; Ubiquitin-Specific Processing Protease 25; Ubiquitin-Specific-Processing Protease 25; Ubi
-
AA Sequence
TVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
