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Vasorin Protein, Human (552a.a, HEK293, His)

Cat. No.: HY-P70999
SDS COA Handling Instructions

Vasorin (VASN) is located in cell surface and extracellular exosome, and may act as an inhibitor of TGF-beta signaling and is involved in negative regulation of epithelial to mesenchymal transition. VASN is also involved in hypoxia and cellular response to redox state, augmenting Notch signaling under hypoxic stress, and expression of VASN is correlated with increased aggression of human gliomas. Vasorin Protein, Human (552a.a, HEK293, His) is the recombinant human-derived Vasorin protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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10 μg $120 In-stock
50 μg $360 In-stock
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Description

Vasorin (VASN) is located in cell surface and extracellular exosome, and may act as an inhibitor of TGF-beta signaling and is involved in negative regulation of epithelial to mesenchymal transition. VASN is also involved in hypoxia and cellular response to redox state, augmenting Notch signaling under hypoxic stress, and expression of VASN is correlated with increased aggression of human gliomas. Vasorin Protein, Human (552a.a, HEK293, His) is the recombinant human-derived Vasorin protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Vasorin (VASN) is located in cell surface and extracellular exosome, containing tandem arrays of a characteristic leucine-rich repeat motif, an epidermal growth factor-like motif, and a fibronectin type III-like motif at the extracellular domain. VASN may act as an inhibitor of TGF-beta signaling and is involved in negative regulation of epithelial to mesenchymal transition.
VASN is also involved in hypoxia and cellular response to redox state, as hypoxia preferentially augments Notch signaling in glioma stem-like cells by inducing VASN, the target gene of HIF1/STAT3. VASN functions as a competitive inhibitor of Numb to reduce Notch turnover, augmenting Notch signaling under hypoxic stress, and expression of VASN is correlated with increased aggression of human gliomas[1][2].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q6EMK4/AAH68575.1 (C24-P575)

Gene ID
Molecular Construction
N-term
Vasorin (C24-P575)
Accession # Q6EMK4/AAH68575.1
6*His
C-term
Synonyms
Vasorin; Protein Slit-Like 2; VASN; SLITL2
AA Sequence

CPSGCQCSQPQTVFCTARQGTTVPRDVPPDTVGLYVFENGITMLDAGSFAGLPGLQLLDLSQNQIASLPSGVFQPLANLSNLDLTANRLHEITNETFRGLRRLERLYLGKNRIRHIQPGAFDTLDRLLELKLQDNELRALPPLRLPRLLLLDLSHNSLLALEPGILDTANVEALRLAGLGLQQLDEGLFSRLRNLHDLDVSDNQLERVPPVIRGLRGLTRLRLAGNTRIAQLRPEDLAGLAALQELDVSNLSLQALPGDLSGLFPRLRLLAAARNPFNCVCPLSWFGPWVRESHVTLASPEETRCHFPPKNAGRLLLELDYADFGCPATTTTATVPTTRPVVREPTALSSSLAPTWLSPTAPATEAPSPPSTAPPTVGPVPQPQDCPPSTCLNGGTCHLGTRHHLACLCPEGFTGLYCESQMGQGTRPSPTPVTPRPPRSLTLGIEPVSPTSLRVGLQRYLQGSSVQLRSLRLTYRNLSGPDKRLVTLRLPASLAEYTVTQLRPNATYSVCVMPLGPGRVPEGEEACGEAHTPPAVHSNHAPVTQAREGNLP

Molecular Weight

Approximately 87.39 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Vasorin Protein, Human (552a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Vasorin Protein, Human (552a.a, HEK293, His)
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HY-P70999
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