Vasorin Protein, Human (552a.a, HEK293, His)

Vasorin (VASN) is located in cell surface and extracellular exosome, and may act as an inhibitor of TGF-beta signaling and is involved in negative regulation of epithelial to mesenchymal transition. VASN is also involved in hypoxia and cellular response to redox state, augmenting Notch signaling under hypoxic stress, and expression of VASN is correlated with increased aggression of human gliomas. Vasorin Protein, Human (552a.a, HEK293, His) is the recombinant human-derived Vasorin protein, expressed by HEK293 , with C-6*His labeled tag.

Vasorin (VASN) is located in cell surface and extracellular exosome, containing tandem arrays of a characteristic leucine-rich repeat motif, an epidermal growth factor-like motif, and a fibronectin type III-like motif at the extracellular domain. VASN may act as an inhibitor of TGF-beta signaling and is involved in negative regulation of epithelial to mesenchymal transition.
VASN is also involved in hypoxia and cellular response to redox state, as hypoxia preferentially augments Notch signaling in glioma stem-like cells by inducing VASN, the target gene of HIF1/STAT3. VASN functions as a competitive inhibitor of Numb to reduce Notch turnover, augmenting Notch signaling under hypoxic stress, and expression of VASN is correlated with increased aggression of human gliomas^[1][2].

Human

HEK293

C-6*His

Q6EMK4/AAH68575.1 (C24-P575, E384A)

114990  [NCBI]

Extracellular Domain

CPSGCQCSQPQTVFCTARQGTTVPRDVPPDTVGLYVFENGITMLDAGSFAGLPGLQLLDLSQNQIASLPSGVFQPLANLSNLDLTANRLHEITNETFRGLRRLERLYLGKNRIRHIQPGAFDTLDRLLELKLQDNELRALPPLRLPRLLLLDLSHNSLLALEPGILDTANVEALRLAGLGLQQLDEGLFSRLRNLHDLDVSDNQLERVPPVIRGLRGLTRLRLAGNTRIAQLRPEDLAGLAALQELDVSNLSLQALPGDLSGLFPRLRLLAAARNPFNCVCPLSWFGPWVRESHVTLASPEETRCHFPPKNAGRLLLELDYADFGCPATTTTATVPTTRPVVREPTALSSSLAPTWLSPTAPATEAPSPPSTAPPTVGPVPQPQDCPPSTCLNGGTCHLGTRHHLACLCPEGFTGLYCESQMGQGTRPSPTPVTPRPPRSLTLGIEPVSPTSLRVGLQRYLQGSSVQLRSLRLTYRNLSGPDKRLVTLRLPASLAEYTVTQLRPNATYSVCVMPLGPGRVPEGEEACGEAHTPPAVHSNHAPVTQAREGNLP

Approximately 66-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.