Vimentin Protein, Human (sf9, His)

Vimentin, a class-III intermediate filament, predominantly exists in non-epithelial cells, particularly mesenchymal cells. This structural protein forms networks linking the nucleus, endoplasmic reticulum, and mitochondria. Teaming up with LARP6, vimentin stabilizes messenger RNAs (mRNAs) of type I collagen, notably CO1A1 and CO1A2, showcasing its role in regulating extracellular matrix components crucial for cellular structure and integrity. Vimentin Protein, Human (sf9, His) is the recombinant human-derived Vimentin protein, expressed by Sf9 insect cells , with C-His labeled tag.

Vimentin, a class-III intermediate filament, is predominantly present in non-epithelial cells, particularly mesenchymal cells. This protein forms a structural network, linking to the nucleus, endoplasmic reticulum, and mitochondria either laterally or terminally. In collaboration with LARP6, vimentin plays a crucial role in stabilizing the messenger RNAs (mRNAs) of type I collagen, specifically CO1A1 and CO1A2, emphasizing its involvement in the regulation of extracellular matrix components essential for cellular structure and integrity.

Human

Sf9 insect cells

C-10*His

P08670 (M1-E466)

7431  [NCBI]

Full Length

MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 40% acetonitrile, 0.1% TFA, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/raw264-7-cell.html  [Content Brief]