Cadherin-1/CD324 Protein, Human (HEK293, His)

Cadherin-1/CD324 Protein, Human (HEK293, His) is a recombinant human E-cadherin produced in HEK293 cells, with His tag. Cadherin-1/CD324/E-cadherin is one of the most important molecules in cell-cell adhesion in epithelial tissues^[1].

Cadherin-1/CD324/E-cadherin/CAM 120/80 is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. As a member of a large family of genes coding for calcium-dependent cell adhesion molecules (CAMs), the cadherin glycoproteins are expressed by a variety of tissues, mediating adhesion through homotypic binding. Classical cadherins-E- and N-cadherins being the best characterized-play important roles in the formation of tissues during gastrulation, neurulation and organogenesis^[1].

Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED₅₀ is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 10³ units/mg.

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  Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED₅₀ is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 10³ units/mg.

Human

HEK293

C-6*His

P12830 (D155-I707)

999  [NCBI]

DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIHHHHHH

80-90 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Nives Pećina-Šlaus, et al. Tumor suppressor gene E-cadherin and its role in normal and malignant cells. Cancer Cell Int. 2003; 3: 17.  [Content Brief]