Copy Link Print Information

Cadherin-1/CD324 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

Cadherin-1/CD324 Protein, Human (HEK293, His) is a recombinant human E-cadherin produced in HEK293 cells, with His tag. Cadherin-1/CD324/E-cadherin is one of the most important molecules in cell-cell adhesion in epithelial tissues.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Human (HEK293, His)
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Human (HEK293, His)
  • Cadherin-1/CD324 Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Cadherin-1/CD324 Protein, Human (HEK293, His) is a recombinant human E-cadherin produced in HEK293 cells, with His tag. Cadherin-1/CD324/E-cadherin is one of the most important molecules in cell-cell adhesion in epithelial tissues[1].

Background

Cadherin-1/CD324/E-cadherin/CAM 120/80 is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. As a member of a large family of genes coding for calcium-dependent cell adhesion molecules (CAMs), the cadherin glycoproteins are expressed by a variety of tissues, mediating adhesion through homotypic binding. Classical cadherins-E- and N-cadherins being the best characterized-play important roles in the formation of tissues during gastrulation, neurulation and organogenesis[1].

Verified Bioactivity

Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 103 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P12830-1 (D155-I707)

  • Gene ID
    999  [NCBI]
  • Molecular Construction
    • N-term
    • CADH1 (D155-I707)
      Accession # P12830-1
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CDH1; CDHE; Prev. UVO; Epididymis Secretory Sperm Binding Protein; Uvomorulin; Cadherin 1, E-Cadherin (Epithelial); Epithelial Cadherin; Cell-CAM 120/80; E-Cadherin; CD324 Antigen; Cadherin-1; Arc-1; CD324; BCDS1; Cadherin 1, Type 1, E-Cadherin (Epithelia

  • AA Sequence

    DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQI

  • Molecular Weight

    Approximately 80-90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Nives Pećina-Šlaus, et al. Tumor suppressor gene E-cadherin and its role in normal and malignant cells. Cancer Cell Int. 2003; 3: 17. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 103 units/mg.

  • Cadherin-1/CD324 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Human (HEK293, His)
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Human (HEK293, His)
  • Cadherin-1/CD324 Protein, Human (HEK293, His) Product Image