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WARS Protein, Human (sf9, His)

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WARS includes isomer 1, isomer 2, T1-TrpRS and T2-TrpRS, and has aminoacylation activity, except T2-TrpRS. Unlike isoform 1, isoform 2, T1-TrpRS and T2-TrpRS, exhibit vasostatic activity. WARS Protein, Human (sf9, His) is the recombinant human-derived WARS protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • WARS Protein, Human (sf9, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

WARS includes isomer 1, isomer 2, T1-TrpRS and T2-TrpRS, and has aminoacylation activity, except T2-TrpRS. Unlike isoform 1, isoform 2, T1-TrpRS and T2-TrpRS, exhibit vasostatic activity. WARS Protein, Human (sf9, His) is the recombinant human-derived WARS protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

WARS, encompassing Isoform 1, Isoform 2, T1-TrpRS, and T2-TrpRS, displays aminoacylation activity, with T2-TrpRS being the exception as it lacks this enzymatic function. In terms of angiostatic activity, Isoform 2, T1-TrpRS, and T2-TrpRS exhibit this property, while Isoform 1 does not possess angiostatic capabilities. Particularly, T2-TrpRS stands out by inhibiting fluid shear stress-activated responses in endothelial cells. WARS plays a pivotal role in regulating key signaling pathways, including ERK, Akt, and eNOS activation, which are associated with angiogenesis, cytoskeletal reorganization, and the expression of genes responsive to shear stress. This multifaceted functionality underscores WARS's involvement in intricate cellular processes and its potential impact on vascular responses.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    P23381 (P2-Q471)

  • Gene ID
    7453  [NCBI]
  • Molecular Construction
    • N-term
    • WARS (P2-Q471)
      Accession # P23381
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    WARS1; Tryptophanyl‑TRNA Synthetase 1; IFI53; WARS; IFP53; Tryptophan‑‑TRNA Ligase, Cytoplasmic; Tryptophanyl‑TRNA Synthetase; Tryptophan TRNA Ligase 1, Cytoplasmic; Interferon‑Induced Protein 53; TrpRS; HWRS; Epididymis Secretory Sperm Binding Protein; T

  • AA Sequence

    PNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ

  • Predicted Molecular Mass

    55.2 kDa

  • Molecular Weight

    Approximately 55 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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