WARS Protein, Human (sf9, His)

WARS includes isomer 1, isomer 2, T1-TrpRS and T2-TrpRS, and has aminoacylation activity, except T2-TrpRS. Unlike isoform 1, isoform 2, T1-TrpRS and T2-TrpRS, exhibit vasostatic activity. WARS Protein, Human (sf9, His) is the recombinant human-derived WARS protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of WARS Protein, Human (sf9, His) is 470 a.a., with molecular weight of ~55 kDa.

WARS, encompassing Isoform 1, Isoform 2, T1-TrpRS, and T2-TrpRS, displays aminoacylation activity, with T2-TrpRS being the exception as it lacks this enzymatic function. In terms of angiostatic activity, Isoform 2, T1-TrpRS, and T2-TrpRS exhibit this property, while Isoform 1 does not possess angiostatic capabilities. Particularly, T2-TrpRS stands out by inhibiting fluid shear stress-activated responses in endothelial cells. WARS plays a pivotal role in regulating key signaling pathways, including ERK, Akt, and eNOS activation, which are associated with angiogenesis, cytoskeletal reorganization, and the expression of genes responsive to shear stress. This multifaceted functionality underscores WARS's involvement in intricate cellular processes and its potential impact on vascular responses.

Human

Sf9 insect cells

C-His

P23381 (P2-Q471)

7453  [NCBI]

Approximately 55 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.