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WEE2 Protein, Human (Active, sf9, His, GST)

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WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • WEE2 Protein, Human (Active, sf9, His, GST) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

Background

WEE2 Protein, an oocyte-specific protein tyrosine kinase, plays a pivotal role in regulating meiosis during both prophase I and metaphase II. Its essential function is highlighted in maintaining meiotic arrest in oocytes during the germinal vesicle (GV) stage, a prolonged quiescent period at dictyate prophase I. WEE2 achieves this by phosphorylating CDK1 at 'Tyr-15,' leading to the inhibition of CDK1 activity and preventing meiotic reentry. Furthermore, WEE2 is instrumental in facilitating metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15.' This action ensures the orderly exit from meiosis in oocytes, promoting pronuclear formation and contributing to the intricacies of reproductive processes.

Verified Bioactivity

The activity of WEE2 is verified by the ADP-Glo method: Incubate WEE2 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.The specific activity is 1327nM(IC50).

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-8*His;N-GST
  • Accession

    P0C1S8 (D2-H567)

  • Gene ID
    494551
  • Molecular Construction
    • N-term
    • 8*His-GST
    • WEE2 (D2-H567)
      Accession # P0C1S8
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    WEE2; WEE2 Oocyte Meiosis Inhibiting Kinase; WEE1B; Wee1-Like Protein Kinase 1B; Wee1-Like Protein Kinase 2; Wee1B Kinase; WEE1 Homolog 2; FLJ16107; WEE1 Homolog 2 (S. Pombe); OZEMA5; OOMD5

  • AA Sequence

    DDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLH

  • Predicted Molecular Mass

    90.5 KDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 5 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • WEE2 Protein, Human (Active, sf9, His, GST) Product Image