WEE2 Protein, Human (Sf9, His, GST)

WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (Sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of WEE2 Protein, Human (Sf9, His, GST) is 566 a.a., .

WEE2 Protein, an oocyte-specific protein tyrosine kinase, plays a pivotal role in regulating meiosis during both prophase I and metaphase II. Its essential function is highlighted in maintaining meiotic arrest in oocytes during the germinal vesicle (GV) stage, a prolonged quiescent period at dictyate prophase I. WEE2 achieves this by phosphorylating CDK1 at 'Tyr-15,' leading to the inhibition of CDK1 activity and preventing meiotic reentry. Furthermore, WEE2 is instrumental in facilitating metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15.' This action ensures the orderly exit from meiosis in oocytes, promoting pronuclear formation and contributing to the intricacies of reproductive processes.

Human

Sf9 insect cells

N-8*His;N-GST

P0C1S8 (D2-H567)

494551

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.