WEE2 Protein, Human (Active, sf9, His, GST)

WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

WEE2 Protein, an oocyte-specific protein tyrosine kinase, plays a pivotal role in regulating meiosis during both prophase I and metaphase II. Its essential function is highlighted in maintaining meiotic arrest in oocytes during the germinal vesicle (GV) stage, a prolonged quiescent period at dictyate prophase I. WEE2 achieves this by phosphorylating CDK1 at 'Tyr-15,' leading to the inhibition of CDK1 activity and preventing meiotic reentry. Furthermore, WEE2 is instrumental in facilitating metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15.' This action ensures the orderly exit from meiosis in oocytes, promoting pronuclear formation and contributing to the intricacies of reproductive processes.

The activity of WEE2 is verified by the ADP-Glo method: Incubate WEE2 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.The specific activity is 1327nM(IC₅₀).

Human

Sf9 insect cells

N-8*His;N-GST

P0C1S8 (D2-H567)

494551

Full Length

DDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLH

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 5 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.