Resiniferatoxin (Synonyms: (+)-Resiniferatoxin)

Name: Resiniferatoxin
Brand: MedChemExpress
SKU: HY-N2333
Rating: 5.0 (10 reviews)

Cat. No.: HY-N2333 Purity: 99.77%: Data Sheet
COA

SDS
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Resiniferatoxin ((+)-Resiniferatoxin), is a selective agonist of transient receptor potential vanilloid 1 (TRPV1) receptor agonist. Resiniferatoxin can be isolated from the Euphorbia resinifera plant. Resiniferatoxin eliminates TRPV1+ primary sensory afferents and blunt cardiac sympathetic afferent reflex for a relatively long period.

For research use only. We do not sell to patients.

CAS No. : 57444-62-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
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Customer Review

Based on 10 publication(s) in Google Scholar

10 Publications Citing Use of MCE Resiniferatoxin

In Vivo Efficacy Study

IHC

RT-PCR

: Resiniferatoxin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 23:e07128. [Abstract]; Representative images of IHC staining of uteri from mice with PGP9.5. Scale bar, 50 μm. Red arrows: positive PGP9.5 staining. Twenty‐four female mice were randomly assigned to four groups (n = 6 per group): 1) Control (vehicle + vehicle), 2) RTX‐C (vehicle + RTX), 3) M (tamoxifen + vehicle), and 4) RTX‐M (tamoxifen + RTX). Sensory denervation was induced by intraperitoneal injection of RTX (Resiniferatoxin) at 150 µg/kg every 5 days, beginning on day 42. All mice were euthanized on day 63.

: Resiniferatoxin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 23:e07128. [Abstract]; RH&E, Masson trichrome staining of mice uteri at Day 63 and the corresponding horizon of multiple IF staining of E‐CAD (green), αSMA (red), and nucleus (DAPI, Blue). The yellow dotted circle showed the location of the ectopic lesion. The black dotted frame was the zoomed‐out version of the middle picture. Twenty‐four female mice were randomly assigned to four groups (n = 6 per group): 1) Control (vehicle + vehicle), 2) RTX‐C (vehicle + RTX), 3) M (tamoxifen + vehicle), and 4) RTX‐M (tamoxifen + RTX). Sensory denervation was induced by intraperitoneal injection of RTX (Resiniferatoxin) at 150 µg/kg every 5 days, beginning on day 42. All mice were euthanized on day 63.

: Resiniferatoxin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 23:e07128. [Abstract]; Twenty‐four female mice were randomly assigned to four groups (n = 6 per group): 1) Control (vehicle + vehicle), 2) RTX‐C (vehicle + RTX), 3) M (tamoxifen + vehicle), and 4) RTX‐M (tamoxifen + RTX). Sensory denervation was induced by intraperitoneal injection of RTX (Resiniferatoxin) at 150 µg/kg every 5 days, beginning on day 42. All mice were euthanized on day 63.

: Resiniferatoxin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 23:e07128. [Abstract]; Relative expression levels of Fn, Col1a1, Acta2 (gene name of αSMA) in Control, RTX-C, M and RTX-M groups detected by qPCR. Twenty‐four female mice were randomly assigned to four groups (n = 6 per group): 1) Control (vehicle + vehicle), 2) RTX‐C (vehicle + RTX), 3) M (tamoxifen + vehicle), and 4) RTX‐M (tamoxifen + RTX). Sensory denervation was induced by intraperitoneal injection of RTX (Resiniferatoxin) at 150 µg/kg every 5 days, beginning on day 42. All mice were euthanized on day 63.

: Resiniferatoxin purchased from MedChemExpress. Usage Cited in: iScience. 2025 Jan 17;28(2):111831. [Abstract]; RTX was dissolved in 2% DMSO with 0.15% Tween 80 in PBS. 4-week-old C57BL/6J male mice were injected s.c. in the flank on consecutive days with three increasing doses of RTX (Resiniferatoxin, 30, 70, and 100 μg/kg). Control mice were treated with vehicle alone. Mice were rested for 4 weeks before experiments.

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View All Isoforms

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
5637	IC₅₀	19.9 μM Compound: 5; RTX	Antiproliferative activity against human 5637 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Antiproliferative activity against human 5637 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 37000154]
CHO	EC₅₀	0.27 nM Compound: 1, RTX	Agonist activity at rat TRPV1 expressed in CHO cells assessed as 45Ca2+ uptake Agonist activity at rat TRPV1 expressed in CHO cells assessed as 45Ca2+ uptake	[PMID: 21111618]
CHO	EC₅₀	0.27 nM Compound: RTX	Agonist activity at rat TRPV1 receptor expressed in CHO cells assessed as calcium uptake Agonist activity at rat TRPV1 receptor expressed in CHO cells assessed as calcium uptake	[PMID: 19135377]
CHO	EC₅₀	0.92 nM Compound: 1, RTX	Agonist activity at human TRPV1 expressed in CHO cells assessed as stimulation of Ca2+ uptake Agonist activity at human TRPV1 expressed in CHO cells assessed as stimulation of Ca2+ uptake	[PMID: 23981530]
CHO	EC₅₀	0.92 nM Compound: 1, RTX	Agonist activity at human TRPV1 expressed in CHO cells assessed as 45Ca2+ uptake after 20 mins by liquid scintillation counting analysis Agonist activity at human TRPV1 expressed in CHO cells assessed as 45Ca2+ uptake after 20 mins by liquid scintillation counting analysis	[PMID: 24321344]
HEK293	EC₅₀	0.019 nM Compound: 1a, RTX	Agonist activity at human recombinant TRPV1 expressed in human HEK293 cells Agonist activity at human recombinant TRPV1 expressed in human HEK293 cells	[PMID: 17046253]
HEK293	EC₅₀	1.9 nM Compound: 1	Induced [Ca2+] influx in Vanilloid receptor subtype 1 expressing HEK 293 cells Induced [Ca2+] influx in Vanilloid receptor subtype 1 expressing HEK 293 cells	[PMID: 11934585]
HEK293	EC₅₀	19 pM Compound: 2a, RTX	Agonist activity at human TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level Agonist activity at human TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level	[PMID: 19951840]
MT4	EC₅₀	22.6 μM Compound: 29	Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay	[PMID: 25970561]
MT4	EC₅₀	26 μM Compound: 29	Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay	[PMID: 25970561]
T-24	IC₅₀	~ 21.4 μM Compound: 5; RTX	Antiproliferative activity against human T24 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Antiproliferative activity against human T24 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 37000154]
Vero	EC₅₀	1.8 μM Compound: 29	Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days	[PMID: 25970561]

In Vitro

Resiniferatoxin causes extremely prolonged channel opening and calcium influx, which results in cytotoxicity to the TRPV1-positive pain fibers or cell bodies^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Resiniferatoxin (2 μg/10 μl; injected intrathecally into the T2/T3 interspace; four weeks after coronary artery occlusion to induce heart failure in rats) significantly and selectively abolishes the afferent markers expression (TRPV1 and calcitonin gene-related peptide) in dorsal horn and reduced overactivated CSNA. Resiniferatoxin significantly reverses the prolongation of action potential duration (APD) and APD alternan, reduces the inducibilities of ventricular arrhythmias^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

628.71

Formula

C₃₇H₄₀O₉

CAS No.

57444-62-9

Appearance

Solid

SMILES

C[C@H]1[C@]23[C@](C=C(C[C@]4([C@@]3([H])C=C(C4=O)C)O)COC(CC5=CC(OC)=C(C=C5)O)=O)([H])[C@]6([H])[C@](C1)(O[C@](O2)(O6)CC7=CC=CC=C7)C(C)=C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (79.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5906 mL	7.9528 mL	15.9056 mL
5 mM	0.3181 mL	1.5906 mL	3.1811 mL
10 mM	0.1591 mL	0.7953 mL	1.5906 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (1.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (284 KB) SDS (641 KB)

COA (265 KB) HNMR (116 KB) RP-HPLC (151 KB)

Handling Instructions (2659 KB)

References

[1]. Brown DC. Resiniferatoxin: The Evolution of the "Molecular Scalpel" for Chronic Pain Relief. Pharmaceuticals (Basel). 2016;9(3):47. Published 2016 Aug 11. [Content Brief]

[2]. Wu Y, et al. Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats. Eur J Pharmacol. 2020;867:172836. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5906 mL	7.9528 mL	15.9056 mL	39.7640 mL
	5 mM	0.3181 mL	1.5906 mL	3.1811 mL	7.9528 mL
	10 mM	0.1591 mL	0.7953 mL	1.5906 mL	3.9764 mL
	15 mM	0.1060 mL	0.5302 mL	1.0604 mL	2.6509 mL
	20 mM	0.0795 mL	0.3976 mL	0.7953 mL	1.9882 mL
	25 mM	0.0636 mL	0.3181 mL	0.6362 mL	1.5906 mL
	30 mM	0.0530 mL	0.2651 mL	0.5302 mL	1.3255 mL
	40 mM	0.0398 mL	0.1988 mL	0.3976 mL	0.9941 mL
	50 mM	0.0318 mL	0.1591 mL	0.3181 mL	0.7953 mL
	60 mM	0.0265 mL	0.1325 mL	0.2651 mL	0.6627 mL

Resiniferatoxin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Resiniferatoxin57444-62-9(+)-ResiniferatoxinTRP ChannelTransient receptor potential channelsventriculararrhythmiasheartfailurecardiacpainInhibitorinhibitorinhibit

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Resiniferatoxin (Synonyms: (+)-Resiniferatoxin)

Customer Review

Resiniferatoxin Related Antibodies

10 Publications Citing Use of MCE Resiniferatoxin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TRP Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Resiniferatoxin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Resiniferatoxin Related Classifications

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